应用图像分析测定卵巢交界性肿瘤的DNA含量

A quantitative study on nuclear DNA content in ovarian borderline cystadenomas

目的 :探讨卵巢交界性上皮性肿瘤的 DNA含量、倍体分析、S期比例等特点 ,及与组织学分级、预后的关系。方法 :通过计算机图像分析仪 (ICM)测定了 2 2例卵巢交界性肿瘤的 DNA含量 ,并以 38例卵巢良恶性囊性上皮性肿瘤为对照 ,进行倍体分析、S期比例、DI测定。结果 :卵巢交界性肿瘤 DI值 1.11± 0 .0 7,S期比例 33.2 1± 13.72 % ,与良恶性组差异均有显著性意义 (P<0 .0 1)。 2 2例交界性肿瘤中 4例为异倍体肿瘤 ,与恶性组差异有显著性意义 (P<0 .0 1)。 3例复发病例与同组比较其 DI值差异有显著意义 (P<0 .0 1) ,异倍体肿瘤多见。结论 :ICM具有方便、可靠的特点 ,通过测定 DI值、S期比例倍体分析可提示卵巢肿瘤的增殖及预后情况 ,评估潜在恶性的卵巢上皮性肿瘤 。

Objective:To study muclear DNA content,DNA ploidy and S phase fraction(SPE) in ovarian borderline cystadenomas(OBC),and the relationship between DNA content,histological grade and prognosis.Methods:Nuclear DNA content,SPF and DNA ploidy were determined quantitatively by computerized image analysis(ICM) in 22 cases of OBC,18 cases of benign ovarian cystadenoma(BOC) and 20 ovarian cystadenocarcinoma(OC).Results:The DNA index(DI) and SPF of OBC were 1.11±0.07 and 33.21±13.72% respectively. There was significant difference compared with benign and malignant(P<0.01). Four of the 22 cases of BOC were aneuploid.The DI in 3 cases of recurrence was significantly higher than other cases.Conclusion:Nuclear DNA content of OBC is a useful marker in reflecting biological behavior and prognosis,and can evaluate the potentially malignant common epithelial ovarian tumor. [FK(WB80011。6]