骨桥蛋白、整合素ανβ3及ILK表达异常在子宫内膜癌中的相关性研究

CORRELATION OF ABNORMAL EXPRESSION OF OSTEOPONTIN,INTEGRIN ανβ3 AND INTEGRIN - LINKED - KINASE IN ENDOMETRIAL CARCINOMA

目的探讨骨桥蛋白(OPN)、整合素ανβ3和ILK在子宫内膜癌的表达情况以及三者表达的相互关系。方法采用免疫组织化学SP法,检测正常子宫内膜组织(NE)、子宫内膜不典型增生组织(AH)及子宫内膜腺癌组织(EC)中的OPN、整合素ανβ3及ILK表达。结果在NE、AH、EC组织中,OPN、整合素ανβ3及ILK的阳性表达率分别为30%、45%、86.7%;15%、40%、75.6%;20%、25%、71.1%。三者的表达从NE到EC逐渐增高(p<0.01),随EC恶性程度的增高,其表达均显著增加,三者在EC中的表达呈正相关性。结论:OPN、整合素ανβ3及ILK在EC中表达均呈阳性,与肿瘤恶性程度呈明显正相关;EC中三者表达呈正相关性,同时检测三者表达水平可能提高子宫内膜癌的检出率及综合预测预后。

[ Abstract] Objective To study correlation of expression of osteopontin（ OPN）, integrin αvβ and integrin - linked - kinase （ILK） in Endometrial Carcinoma. Methods OPN, αvβ and ILK were detected by immunohisto- chemisty method in normal endometrium（NE） （n = 20）, endometrial atypical hyperplasia（AH） （n = 20 ） and endo- metrial carcinoma（ EC ） （ n = 45 ）. The correlation of their expression and clinical - pathological characteristics were e- valuated. Results The positive percent of OPN,αvβ and ILK in NE, AH and EC were 30%, 45% and 86. 7% ; 15%, 40% and 75. 6% ; 20%, 25% and 71.1% , respectively. The scores of immunostaining increased signifi- cantly from NE to the EC（p 〈 0. 01 ）. The levels of their expression were positively related to the malignant grade of tumor. Conclusion The expressions of OPN, integrin ot αvβ and ILK had a significantly positive correalation. Detec- tion of three markers may help improve detection rate and prognosis of EC.