肺炎患者支气管肺泡洗液中激肽形成机制的探讨

Kinins generation pathway in bronchoalveolar lavage fluid from patients with pneumonia

目的 :探讨下呼吸道急性炎症时激肽生成途径。方法 :测定肺炎患者 (肺炎组 )和健康志愿者 (对照组 )支气管肺泡灌洗液 (BALF)中激肽 ,激肽形成酶活性 ,血浆血管舒缓素 (PK )和α2 巨球蛋白 (α2 M ) ,并鉴定激肽形成酶活性分子来源。结果 :肺炎组BALF中的各种检测指标均明显高于对照组 (P <0 0 0 1) ;经凝胶过滤和酶抑制试验鉴定 ,肺炎患者BALF中主要激肽形成酶活性位于高分子量部分 ,PK抑制物能显著抑制此活性。结论 :肺炎患者激肽和激肽形成活性明显升高 ,其激肽形成酶活性主要来自PK。

Objective: To investigate the kinins generation pathway in acute airway inflammation Methods: The bronchoalveolar lavage fluid (BALF) from patients with pneumonia and healthy individuals was collected to determine kinins, plasma kallikrein, a\-2 macroglobulin and kinins generation enzyme activity Results: Kinins and kinins generation activity in the BALF were significantly higher in patients with pneumonia than in the controls (P<0 001) Gel filtration and enzyme activity inhibition test showed that the kinins generation activity in BALF from patients with pneumonia was inhibited by plasma kallikrein Conclusion: Kinins and kinins generation activity in acute airway inflammation are significantly increased and kinins seems to be mainly generated by plasma kallikrein