梅毒血清固定与外周血调节性T细胞及血清IL-6、IL-10、IFN-γ的关系探讨

Relationship between regulatory T Cells,the Levels of IFN-γ,IL-10 and the sero-resistant syphilis

目的:探讨调节性T细胞的变化与白细胞介素10、6(IL-6,10)、干扰素-γ(IFN-γ)在梅毒血清固定机制中的作用。方法:流式细胞仪测定112例梅毒血清固定患者,155例梅毒血清转阴患者和58例正常人的外周血调节性T细胞及其特异性因子Foxp3的表达;酶联免疫吸附试验(ELISA)方法检测其血清IL-6,IL-10和IFN-γ的水平。结果:梅毒血清固定患者外周血CD4+的表达为(1.95±0.66%),而其中调节性T细胞比例为(24.5±7.63%),较两组对照组均显著升高(P<0.01),CD4+T细胞内转录因子Foxp3表达量(2272.7±876.8)明显高于两组对照组(P<0.01);与对照组相比,梅毒血清固定患者IL-6、IFN-γ水平较低而IL-10水平较高(P<0.01);血清转阴患者与健康献血者三者水平差异无显著性(P>0.05)。结论:调节性T细胞比例的升高以及Th1/Th2比例失衡可能导致了梅毒血清固定。

Objectives： To investigate the role of regulatory T cells and IL- 10）, IL- 6, IFN -γ in the pathogenesis of seroresistant syphilis. Methods： Flow cytometry was used to detect regulatory T cells subsets in peripheral blood and quantify the expression of Foxp3 in CD4＋ T cells; ELISA method was used to detect the serum level of IL- 10, IL-6, IFN-γ in ll2 patients with seroresistant syphilis and 155 patients with negative results after treatment and 58 healthy controls. Results： The ex- pression of CD4 ＋ T cells was 1.95 ±0. 66% in peripheral blood in syphilis patients, increasing more significantly than that in the two controls （ P 〈 0. 01 ）. The expression of Foxp3 of CD4＋ T cells in seroresistant syphilis was higher than that in other two con- trols. The level of IL- l0 was higher, but the level of IL-6 and IFN-γ was lower, compared with those of the controls （P 〈 0.05 ）. However, there was no difference in the levels of all the above indexes between the two controls. Conclusions： The ab- normality of the proportion and the function of regulatory T cells may play a prominent role in the pathogenesis of seroresistant syphilis.