己酮可可碱对肺缺血再灌注后粘附分子表达的影响

Effects of pentoxifylline on adhesion molecules expression in lung ischemia-reperfusion injury in rats

目的 探讨己酮可可碱对肺缺血再灌注损伤后中性粒细胞 (PMN)表面CD18、肺组织ICAM 1表达的影响。方法 72只大鼠随机化方法分为假手术组、缺血再灌注组、PTX组。采用肺在体温缺血再灌注损伤模型 ,于缺血 4 5min、再灌注 1、2、4h测定肺组织含水量、支气管肺泡灌洗液 (BALF)白蛋白含量、肺组织及BALF髓过氧化物酶 (MPO)活性、流式细胞仪测定CD18 FITC标记的PMN阳性细胞百分率 ,肺组织ICAM 1免疫组化染色 ,并进行图像分析。结果 缺血再灌注组再灌注后各时相点肺含水量、BALF白蛋白含量、左肺组织及BALFMPO活性及CD18、ICAM 1表达均有非常显著的升高 (P <0 .0 1) ,PTX则可以明显抑制这种变化。结论 PTX可能通过降低肺缺血再灌注引起的CD11 CD18及ICAM 1的表达 ,而减轻PMN在肺内的聚集、活化 ,从而防治肺缺血再灌注损伤。

Objective To study the effect of pentoxifylline on the expression of the CD18 and ICAM 1 after lung ischemia/reperfusion injury in rats. Methods The rat model of lung ischemia was estaleished with occlusion of left pulmonary hilus for 45 min. Seventy two rats were divided into 3 groups: ① Sham operation group (GroupⅠ, n =24); ②Ischemia reperfusion injury group (GroupⅡ, n =24); ③Pentoxifylline treatment group (GroupⅢ, n =24). Pentoxifylline was injected intravenously 5 min before and 45 min after normothermic ischemia. The changes of water content of lung, MPO and BALF albumin were studied 1 h, 2 h, 4 h during reperfusion after 45 min pulmonary ischemia. Flow cytometric analysis and immunohistochemistry were used to assess the expression of the CD18 on the polymorphonuclear neutrophil(PMN) and ICAM 1 on lung tissue. Results In groupⅡ, water content of lung, MPO and BALF albumin were increased evidently ( P <0.05), the expression of CD18 and ICAM 1 were up regulated. In groupⅢ, the changes were ameliorated as compared with groupⅡ. Conclusion Pentoxifylline can reduce the aggregation and activation of PMNs in the lungs through its effect to inhibit the expression of CD11/CD18 and ICAM 1 after pulmonary ischemia reperfusion. Consequently pulmonary ischemia repefusion injury is prevented.