哌立福辛抑制胃癌细胞SGC7901增殖及其作用机制

Inhibition of perifosine on proliferation of gastric cancer cell line SGC7901 in vitro

目的:探讨哌立福辛对体外培养的人胃癌SGC7901细胞生长增殖的影响并探讨其机制。方法:分别用不同浓度的哌立福辛(0.125、0.250、0.500、0.750、1.500μmol/L)对SGC7901细胞进行干预后,采用磺酰罗丹明B(SRB)法检测细胞增殖变化;平板克隆实验检测细胞克隆形成能力;Real-time PCR、Western blot法检测癌症相关基因eIF4E及AEG-1 mRNA及蛋白表达。结果:哌立福辛呈时间及浓度依赖性抑制胃癌SGC7901细胞的增殖,并降低细胞的克隆形成能力;细胞内AKT信号通路中AEG-1、eIF4E的表达明显降低。结论:哌立福辛具有抑制胃癌SGC7901细胞增殖的作用;通过降低AKT信号通路中AEG-1和eIF4E的mRNA及蛋白表达可能是发挥增殖抑制作用的机制之一。

Objective：To investigate the effects of perifosine on the growth of human gastric carcinoma cell line SGC7901.Methods：The inhibitory effect of perifosine on the growth of human gastric cancer cell line SGC7901 was observed at different concentrations（0.125,0.250,0.500,0.750,1.500） μmol/L by sulforhodamine B（SRB）assay.Plate colony formation assay was performed to detect the colony formation rate.The expression levels of eIF4E and AEG-1 were determined by Real-time PCR and Western blot.Results：Perifosine inhibited the growth of gastric cancer cell line SGC7901 in dose-and time-dependent manners.The cell colony formation rate was statistically decreased（P 0.05） as compared with those in the control.The mRNA and protein levels of eIF4E and AEG-1 were reduced gradually dose-dependently.Conclusion：Perifosine,an AKT inhibitor,inhibits the growth and the colony formation rate of human gastric cancer cell via downregulating the expression of eIF4E and AEG-1.