CXCR4、hTERT表达与胶质瘤微血管密度及预后的关系

Expression of CXCR4,hTERT and its correlation with microvascular dense and prognosis in glioma

目的:探讨趋化因子受体4(CXCR4)、端粒酶逆转录酶(hTERT)在胶质瘤中的表达与肿瘤微血管密度(MVD)及预后的关系。方法:应用免疫组织化学技术检测156例胶质瘤中CXCR4及hTERT的表达,以血管内皮生长因子(VEGF)标记检测微血管密度,分析CXCR4、hTERT表达及MVD与生存时间的关系。结果:①CXCR4、hTERT总阳性率分别为53.8%(84/156)、50.6%(79/156);Ⅱ、Ⅲ、Ⅳ级胶质瘤中CXCR4的阳性率分别为30.8%(16/52)、56.5%(39/69)、82.9%(29/35),hTERT的阳性率分别为26.9%(14/52)、53.6%(37/69)、80.0%(28/35),CXCR4和hTERT在不同级别胶质瘤中的表达差异有统计学意义(P<0.01);②156例胶质瘤中MVD均值为44.17±13.43,Ⅱ、Ⅲ、Ⅳ级MVD均值分别为29.85±4.34、50.12±17.80、52.53±18.16(P<0.01);③Ⅱ、Ⅲ、Ⅳ级胶质瘤患者术后中位生存时间分别为30.2、11.7、9.8个月;CXCR4、hTERT阳性表达组中MVD≥44.17的病例术后中位生存时间明显短于阴性表达组及MVD<44.17的病例(P<0.01)。结论:CXCR4、hTERT的高表达可促进胶质瘤血管生成和侵袭性生长,三者联合检测有助于预后评估。

Objective：To investigate the expressions of CXCR4,hTERT and microvascular density（MVD） in gliomas and discuss their relationships with the prognosis of glioma.Methods：Immunohistochemistry was used to detect the expression of CXCR4 and hTERT in 156 human gliomas,MVD was analyzed by VEGF labeling.Their relationships with survival time of patients were also analyzed.Results： ① The positive rates of CXCR4 and hTERT in gliomas were 53.8%（84/156） and 50.6%（79/156）,respectively.The positive rates of CXCR4 and hTERT in grade Ⅱ,grade Ⅲ and grade Ⅳ of gliomas were 30.8%（16/52）,56.5%（39/69）,82.9%（29/35）,and 26.9%（14/52）,53.6%（37/69）,80.0%（28/35） respectively.A statistically significance of their expressions was observed among different grades（P 0.01）.② The average of MVD in 156 gliomas was 44.17 ± 13.43.In grade Ⅱ,grade Ⅲ and grade Ⅳ of gliomas,MVD was 29.85 ± 4.34,50.12 ± 17.80 and 52.53 ± 18.16,respectively（P 0.01）.③ The median survival time of patients with gradeⅡ,Ⅲ,Ⅳ of gliomas after operations was 30.2,11.7 and 9.8 months,respectively.The median survival time in cases with positive expression of CXCR4,hTERT or MVD ≥44.17 were significantly shorter than that in cases with negative expression or MVD 44.17（P 0.01）.Conclusion：The expressions of CXCR4,hTERT and MVD may be associated with invasive group and tumor angionenesis,their combined assay is helpful for evaluating prognosis.