摘要
目的:建立生活饮用水中氯硝柳胺的高效液相色谱测定方法。方法:应用高效液相色谱,C18色谱柱,以甲醇:水(85:15)为流动相,流速1.0 ml/min。二极管阵列检测器在330 nm波长下测定吸收值。结果:线性范围0.2 mg/L^25 mg/L,相关系数r=0.9999,最低检出浓度为0.3μg/L,三个浓度水平的样品加标回收率为95.02%~103.9%,相对标准偏差RSD均<5%。结论:该方法线性范围宽,相关性好,精密度高,准确度好,特异性强。
Objective： To establish a new method for the determination of niclosamide in drinking water by HPLC. Methods： The separation was carried out on an Cls column by using the mobile phase consisting of CH3OH ＋ H2O（85 ＋ 15）. The flow rate was 1.0 ml/min. Detection was done by DAD at the wavelength of 330 nm. Results： The linearity was obtained in the range of 0.2 mg/L -25 mg/L, the correlation coefficient was 0. 9999. The limit of detection was 0.3 μg/L, the rate of recovery was between 95.02% - 103.9% with RSD less than the reference value 5. Conclusion： This method has good accuracy and precision. This method is suitable for the determination of niclosamide in drinking water.
出处
《中国卫生检验杂志》
北大核心
2013年第7期1720-1722,共3页
Chinese Journal of Health Laboratory Technology
基金
卫生标准(修)订项目(20110101-14)
