葛根正丁醇部位的化学成分研究

Chemical constituents in n-butanol fraction of Puerariae Radix

目的研究葛根正丁醇萃取物的化学成分。方法采用聚酰胺柱色谱、反相ODS柱色谱、凝胶柱色谱、制备液相色谱等方法进行分离纯化,根据化合物的理化性质及波谱数据鉴定其结构。结果分离鉴定了12个化合物,分别为葛酚苷元A(1)、葛酚苷元B(2)、染料木素(3)、3′-甲氧基葛根素(4)、3′-羟基葛根素(5)、葛根素芹糖苷(6)、葛根素木糖苷(7)、大豆苷元(8)、大豆素7,4′-二葡萄糖苷(9)、大豆苷(10)、葛根素(11)、胡萝卜苷(12)。结论其中化合物1、2为首次从葛属植物中分离得到。

Objective To study the chemical constituents in n-buytl alcohol fraction of Puerariae Radix. Methods The compounds were isolated by Polyamide, ODS, and Sephadex LH-20 column chromatographies, and preparative HPLC, and their structures were identified by spectral analyses and physiochemical properties. Results Twelve compounds were isolated and identified as puerol A （1）, puerol B （2）, genistein （3）, 3′-methoxy puerarin （4）, 3′-hydroxyl puerarin （5）, mirificin （6）, puerarin xylosidde （7）, daidzein （8）, daidzein 7,4′-diglucoside （9）, daidzin （10）, puerarin （11）, and daucosterol （12）. Conclusion Compouds 1—2 are isolated from the plants of Pueraria DC. for the first time.