何首乌不同炮制品对肝细胞脂代谢的干预作用研究

Intervention effects of Polygoni Multiflori Radix and its processed products on lipid metabolism in steatosis hepatocyte L-02

目的利用脂肪变性肝L-02细胞模型,比较何首乌的生品及不同炮制品(传统方法炮制品、发酵炮制品)对脂代谢的干预作用。方法将肝细胞传板至6孔板,分为正常组、模型组、给药组细胞。模型组与给药组采用含医用脂肪乳的培养液诱导48h,建立肝细胞脂肪变性模型,给药组给予何首乌生品及不同炮制品的各浓度水提取物、50%乙醇提取物刺激液进行培养,采用洛伐他汀、非诺贝特为阳性对照。24h后测定肝细胞内三酰甘油(TG)、胆固醇(TC)的量。结果与模型组相比,何首乌米根霉发酵品水提取物及50%乙醇提取物、生何首乌水提取物、黑豆制何首乌50%乙醇提取物和清蒸何首乌50%乙醇提取物在该模型上具有较好的降脂作用。其中何首乌发酵炮制品的降脂活性尤其明显,何首乌米根霉发酵品能极显著地降低TG的量(P<0.01)至模型组含量的25%;何首乌米根霉发酵品水提取物和黑曲霉发酵品的50%乙醇提取物均可降低TC的量(P<0.01)至模型组含量的35%。结论生何首乌及何首乌传统炮制品、发酵炮制品均有显著地降脂作用,其中由米根霉发酵得到的发酵品表现出的较好的降脂活性,表明发酵法可能成为何首乌炮制法研究的新方向。

Objective To investigate the intervention effects of Polygoni Multiflori Radix （PMR） and its traditional processed and fermented products on the lipid metabolism of steatosis hepatocyte. L-02. Methods The hepatocytes were cultured in 6-well plates and divided into control, model, and treatment groups. The steatosis hepatocyte L-02 model was established by exposure in fat emulsion for 48 h. The hepatocytes in the treatment group were treated with the water extracts and 50% ethanol extract of PMR and its processed products by different way, and lovastatin and fenofibrate were used as positive control. The triacylglycerol （TG） and cholesterin （TC） contents were determined after 24 h. In the same time those were studied in groups. Results Compared with the model group, the water extract and 50% ethanol extract of PMR fermented with Rhizopus oryzae, water extract of PMR, 50% ethanol extract of PMR processed by black bean, and 50% ethanol extract of steaming PMR showed great lipid-lowering effects. PMR fermented with R. oryzae could even significantly reduce the content of TG to about 25% and the content of TC to about 35% in the model group, similar to PMR fermented with Aspergillus niger. Conclusion PMR and its traditionally processed and fermented products show the lipid-lowering effect on TC and TG, especially PMR fermented by R. oryzae. These results indicate the fermentation method may be a new research processing method of PMR.