大孔吸附树脂对苦豆子多糖纯化工艺研究

Purification technology of Sophora alopecuroides polysaccharides by macroporous adsorption resin

目的筛选纯化苦豆子多糖的最佳大孔树脂并确定纯化多糖的最佳工艺条件。方法采用静态吸附分离法对树脂进行筛选,并以苦豆子多糖的纯度和回收率为考察指标,对筛选出的树脂进行工艺优化。结果 AB-8树脂对苦豆子多糖有较好的吸附纯化性能,最佳纯化条件为:上样多糖溶液质量浓度为5mg/mL,pH值为7.0,温度为25℃,纯化后的苦豆子多糖的质量分数达到88.90%,比纯化前提高了17.60%,回收率90.62%。结论 AB-8树脂适用于苦豆子多糖的分离纯化,该方法不仅稳定有效,而且具有较高的多糖回收率。

Objective To optimize the macroporous resin and technologcial conditions for the purification of Sophora alopecuroides polysaccharides. Methods The static adsorption and separation method was used in the optimization of macroporous adsorption resin. The technological process was compared and evaluated in terms of the ratio of polysaccharide purity and coefficient of recovery. Results The resin AB-8 had the best performance of adsorption and desorption effects and was selected to purify S. alopecuroides polysaccharides. The optimum technology parameters were as follows： the sample concentration of 5 mg/mL, pH 7, and 25 ℃. The purity of polysaccharides was 88.90%, improved more 17.60% than that of crude polysaccharide, and the coefficient of recovery was 90.62% by using the above technology. Conclusion The resin AB-8 is a preferable candidate for the isolation and purification of S. alopecuroides polysaccharides, with the better stability and effectiveness and higher recovery.