Survivin启动子驱动LRIG1基因表达的重组腺病毒对人膀胱癌细胞T24侵袭能力的影响

Effect of recombinant adenoviral vector carrying LRIG1 gene driven by Survivin promoter on the invasion of human bladder cancer cells T24 in vitro

目的以Survivin启动子驱动LRIG1基因表达的重组腺病毒Ad-Surp-LRIG1转染人膀胱癌细胞系T24细胞,观察其对T24细胞侵袭能力的影响。方法 Ad-Surp-LRIG1转染T24细胞,用RT-PCR和Westernblot检测细胞中LRIG1的表达水平,并采用Transwell小室测定转染后细胞侵袭能力的变化。结果 Ad-Surp-LRIG1转染人膀胱癌T24细胞后,与对照组和空白对照组相比,体外侵袭实验显示实验组细胞的侵袭能力明显下降。LRIG1的mRNA和蛋白表达水平明显升高,而EGFR的mRNA和蛋白表达水平则显著降低(P<0.01)。结论 Survivin启动子驱动LRIG1基因表达的重组腺病毒Ad-Surp-LRIG1能够在T24细胞中有效表达,并显著抑制T24细胞的侵袭能力。

Objective To study the effect of invasion ability of the bladder cancer cell line T24 after transfeeted with recombinant adenoviral vector carrying LRIG1 gene driven by Survivin promot- er （Ad-Surp-LRIG1）. Methods Ad-Surp-LRIG1 was infected into bladder cancer cells T24, the expression of mRNA and protein of LRIG1 and EGFR were detected by RT-PCR and Western blot. And Transwell Chamber were used to assay the change of invasion ability of transfected cell lines in vitro. Results After T24 cell line was infected with Ad-Surp-LRIG1, the invasion ability de- creased significantly, the expression of mRNA and protein of LRIG1 were obviously up-regulated and those of EGFR were down-regulated （P〈0. 01）. Conclusions Ad-Surp-LRIG1 can signifi- cantly inhibit the invasion ability of T24 cell line.