复方丹参片中三七总皂苷的含量测定

Determination of Panax Notoginseng and Salviae Miltiorrhizae in Compound Danshen Tablets by HPLC

目的:建立复方丹参片中三七皂苷R1、人参皂苷Rb1及人参皂苷Rg1含量测定方法。方法:采用HPLC法,用Diamonsil C18(250 mm×4.6 mm,5μm)色谱柱,乙腈-0.05%磷酸溶液为流动相。检测波长为203 nm,流速为1.0 mL/min,进样量为10μL。结果:三七皂苷R1线性回归方程Y=187.0X+16.16,r=0.9997,三七皂苷R1含量在0.366-3.206μg之间为良好线性关系。人参皂苷Rb1含量在0.911-9.241μg之间为良好线性关系。人参皂苷Rg1线性回归方程Y=403.6X+9.113,r=0.9994,人参皂苷Rg1含量在0.841-8.431μg之间为良好线性关系。结论:该法可以用于测定复方丹参片中三七皂苷R1、人参皂苷Rb1及人参皂苷Rg1含量,可以为药物的质量控制提供参考。

Objective： To establish the method to determine the content of Notoginsenoside R1,Ginsenoside Rb1 and Ginsenoside Rg1 in Compound Danshen Tablets by HPLC.Methods： The HPLC method was applied and Diamonsil C18（250 mm×4.6 mm,5 μm） was used;the ACN-H3PO4（0.05%） was used as mobile phase;detective wave was 203 nm;flow rate was 10 mL/min.Results：The regression equations of notoginsenoside R1 was Y=187.0X＋16.16,r=0.9997,and the linear range of notoginsenoside R1 was 0.366-3.206 μg;the linear range of ginsenoside Rb1 was 0.911-9.241 μg;the regression equations of ginsenoside Rg1,was Y =403.6X ＋9.113,r =0.9994,and the linear range of ginsenoside Rg1 was0.841-8.431 μg.Conclusion： The HPLC is promise method to determine the content of Notoginsenoside R1,Ginsenoside Rb1 and Ginsenoside Rg1,which is benefit to control the quality of Compound Danshen Tablets.