多重耐药鲍曼不动杆菌碳青霉烯酶基因型研究

Carbapenemase genotype among multi-drug resistant Acinetobacter baumannii

目的研究多重耐药鲍曼不动杆菌对亚胺培南的耐药性及其携带的碳青霉烯酶基因型。方法琼脂二倍稀释法测定亚胺培南对多重耐药鲍曼不动杆菌的MIC;PCR检测与碳青霉烯类抗菌素耐药相关的基因,并分析与耐药程度的相关性。结果 76株多重耐药鲍曼不动杆菌有65株对亚胺培南耐药,65株亚胺培南耐药株OXA-23、OXA-51和OXA-58阳性率分别为96.9%、98.5%、1.5%。结论本院多重耐药鲍曼不动杆菌对亚胺培南耐药严重,OXA-23和OXA-51是主要的碳青霉烯酶基因型。

Objective To study the imipenem resistance and carbapenemase genotype of multi-drug resistant Acinetobacter baumannii.Methods The MIC of imipenem was determined by 2-fold agar dilution method followed by NCCLS recommendations.The genes associated with carbapenemase were amplified by PCR.Results There were 65 strains resistant to imipenem among 76 multi-drug resistant Acinetobacter baumannii.The positive rate of OXA-23、OXA-51 and OXA-58 in 65 imipenem-resistant Abaumannii were 96.9%、98.5% and 1.5%.Conclusion The multi-drug resistant Acinetobacter baumannii have a high resistant rate to imipenem and OXA-23 and OXA-51 type carbapenemase are the major carbapenemase that contributes to the nosocomial infection of Acinetobacter baumannii.