武汉地区丙型肝炎病毒基因分型与慢性丙型肝炎患者肝功能和血液指标分析

Patients with hepatitis C for genotyping,liver function and blood analysis in Wuhan area

目的了解武汉地区慢性丙型肝炎患者基因型分布特点,分析HCV不同基因型患者肝功能及血液学指标的变化。方法应用逆转录-聚合酶链反应(RT-PCR)技术对临床已诊断为丙肝患者(病例组)进行HCVRNA载量检测,并使用PCR直接测序法进行HCV基因分型,同时检测每位患者肝功能和血液学指标。另选90例健康体检者(对照组)进行相同检测。结果 90例慢性丙肝患者中以HCV1b型和2a型为主,分别为74例(82%,RNA载量对数值为8.19±2.33)和7例(7.2%,RNA载量对数值为6.12±1.56)。患者肝功能指标高于对照组,血液学指标低于对照组(P<0.05或P<0.01),但不同基因型患者肝功能和血液学指标测值未见明显差异(P>0.05)。结论武汉地区HCV基因分型及慢性丙肝患者肝功能、血液学检查对临床治疗有重要意义。

Objective To study the distribution characteristics of Wuhan area in patients of hepatitis C,analysis the change of liver function and blood indexes in different HCV genotype.Methods Quantitative detection of HCVRNA for the clinical diagnosis of hepatitis C patients（the case group）,and using reverse transcription-polymerase chain reaction（RT-PCR）.Direct sequencing for HCV genotyping and each patient were detected in liver function and blood analysis.Another 90 healthy subjects（control group） were the same detection.Results In 90 patients,there were HCV1b 74 cases（82%,HCV RNA 8.19±2.33）and 2a 7 cases（7.2%,HCV RNA 6.12±1.56）.The liver function of case group was higher than control group and blood indexes were lower（P0.05）.But the liver function and blood indexes of different HCV genotype had no significant difference（P0.05）.Conclusion The genotype,liver function and blood indexes in patients of hepatitis C in Wuhan area has important clinical significance in clincal treatment.