低剂量电磁辐射对小鼠肝脏功能的影响研究

Effect of low dose electromagnetic radiation on liver function in mice

目的探讨低剂量电磁辐射后不同时间段的小鼠肝脏功能指标变化情况。方法将36只健康无特定病原体SPF级Winster雄性小鼠随机分为3组,分别接受功率密度为0mW/cm2、10mW/cm2和20mW/cm2的电磁波辐照60min,辐照结束后于1h,1、3、7、15、30d分别取小鼠尾静脉血和眼眶血进行血常规和肝功能检测。结果与对照组(0mW/cm2)相比,功率密度为10mW/cm2和20mW/cm2的电磁辐射照射后小鼠的白细胞、红细胞随着时间增加,血小板总数则随时间而显著减少(P<0.05)。同时,其丙氨酸转氨酶(ALT)、天门冬氨酸氨基转移酶(AST)、γ-谷氨酰转移酶(GGT)亦随时间同步增加(P<0.05)。对于相同指标,功率密度为20mW/cm2的一组所产生的影响要高于10mW/cm2组(P<0.05)。结论低剂量电磁辐射对生物体的肝脏损伤随着时间的增加而增强,且电磁辐射功率的密度强弱与其对机体的损伤层正相关。

Objective To investigate the effect of low dose electromagnetic radiation on mice liver function.Methods 36 healthy SPF Winster male mice were divided into three groups,and each of them was irradiated for 60 min using 0 mW/cm2,10 mW/cm2 and 20 mW/cm2 radiation.Then the tail venous blood and orbital blood were taken for routine blood test and liver function examination at 1 h,1,3,7,15 and 30 d after irradiation.Results Compared with control group,the power density of 10 mW/cm2 and 20 mW/cm2 of electromagnetic radiation induced the increase of white blood cells and plateles in mice（P0.05）.And alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,and gamma-glutamine transferase（GGT） also increased with the time interval,with a peak at the 3 d after irradiation（P0.05）.In addition,20 mW/cm2 group caused more serious effect on the biochemical test than 20 mW/cm2 group（P0.05）.Conclusion Even low dose electromagnetic radiation has significant effect on the organism′s blood cell counts and liver function,and the damage extent is proportional to the power density of electromagnetic radiation.