宫颈病变中人乳头瘤病毒感染特征分析

Analysis the prevalence and type distribution of human papillomavirus in cervical lesions

目的探讨人乳头瘤病毒(HPV)在妇女宫颈病变中的型别分布特征及感染特点。方法收集2009～2011年1 213例宫颈病变活检组织标本(CINⅠ317例,CINⅡ233例,CINⅢ208例,宫颈鳞状细胞癌198例,慢性宫颈炎257例),采用PCR-反向点杂交法进行HPV DNA分型检测。结果 HPV在女性宫颈病变中的平均感染率为69.74%,随着病变严重程度的增加HPV感染率亦增加,二者呈正相关(P<0.05)。各组病变中以HPV16的感染率最高,其次为HPV58、18、33、11。CINⅠ组中感染最多的前3位为HPV16、58、11;CINⅡ组中感染最多的前3位为HPV16、58、33;CINⅢ组中感染最多的前3位为HPV16、58、18;SCC组中感染最多的前3位为HPV16、18、58;宫颈炎组中感染最多的前3位为HPV16、58、33。每组病变都存在单纯感染和复合感染,复合感染随着病变严重程度的增加而减少。结论 HPV的感染率及型别分布在不同病变的宫颈组织中均有一定的差异,致癌性肯定的HPV亚型中感染率较高的为HPV16、18和58。

Objective To explore the prevalence and subtype distribution of human papillomavirus（HPV） in cervical lesions.Methods From 2009 to 2011,1213 specimens,included 257 cases of chronic cervicitis,758 cases of CIN,and 198 cases of cervical squamous cell carcinoma（SCC）,were collected for this study.PCR-reverse dot blot was adopted for HPV-DNA testing.Results The positive rate of HPV was 69.74% and was increased along with the severity of cervical lesions in all cases.The HPV l6 was the most common subtype,followed by HPV58、18、33、11.The top three HPV subtype were HPV16、58、11 in CIN Ⅰ group,were HPV16、58、33 in CIN Ⅱ group,were HPV16、58、18 in CIN Ⅲ group,were HPV16、18、58 in SCC group and were HPV16、58、33 in chronic cervicitis group.Single and composite infection were in all groups.The rate of composite infection was negatively correlated with the degree of cervical lesions.Conclusion HPV infection rate and distribution vary in various lesions of cervical tissue.HPV 16,18 and 58 were the most common types in carcinogenic HPV genotypes.