转录因子8在肿瘤血管发生过程中作用初探

Role of Transcription Factor 8 in the Regulation of Tumor Angiogenesis

目的研究转录因子8(TCF8)在肿瘤血管生成过程中的作用。方法通过siRNA转染敲除人脐静脉血管内皮细胞(HUVECs)中的TCF8,通过实时PCR和Weston blot检测转染后的HUVECs中TCF8 mRNA和蛋白的表达情况,并通过体外小管形成实验观察TCF8被敲除后对HUVECs管腔形成能力的影响。结果siTCF8转染HUVECs 72 h后TCF8的RNA和蛋白表达水平siTCF8组明显低于sicontrol组(P<0.05);体外小管形成实验显示siTCF8组形成后的多数管腔不完整,其完整管腔数量形成明显少于sicontrol组(P<0.05)。结论应用RNA干扰技术可有效干扰TCF8的表达,敲除TCF8可以抑制肿瘤血管形成,TCF8有可能成为临床上抗肿瘤血管生成治疗的新靶点。

Objective To investigate the role of transcription Factor 8 （TCF8） in the regulation of tumor angiogenesis. Methods TCF8 of the human umbilical vascular endothelial ceils （HUVECs） was knocked down by siRNA. The RNA and protein expressions of TCF8 were detected in HUVECs which were transfected by real-time PCR and Western blot. And the role of TCF8 in tumor angiogenesis was observed by the tube formation assay. Results The RNA and protein expressions of TCF8 in the siTCF8 group were lower than those of the sicontrel group （ P 〈 0.05 ）. The vasculatures of the siTCF8 group were unsettled and leaky, and the number of the settled vasculatures was lower than that of the sicontrol group by the tube formation assay （ P 〈 0.05 ）. Conclusion RNA interference can effectively inhibit the expression of TCF8, knocking down TCF8 in endothelial cells can depress the formation of new tumor vessel, suggesting that TCF8 might be a good target for the anti-angiogenesis treatment of cancer in clinical medicine.