食管癌DNA倍体分析方法探讨

Method Study of DNA Ploidy Analysis of Esophageal Cancer

目的探讨不同方法检测DNA倍体结果的一致性及可靠性。方法分别采用单细胞制备仪、组织匀浆器及石蜡组织提取DNA标本,通过流式细胞仪同时检测56例食管癌DNA倍体。结果采用单细胞制备仪、组织匀浆器及石蜡组织提取DNA标本,56例食管癌非整倍体率分别为48.21%、44.64%和42.86%,其中各有1例为近二倍体。采用石蜡组织法检测DNA倍体SPF值为(18.205±7.719)%,高于组织匀浆器提取法的(17.330±8.160)%和单细胞制备仪提取法的(15.479±7.811)%,但差异无统计学意义(t=0.583、1.858,P=0.561、0.066);组织匀浆器提取法SPF值高于单细胞制备仪提取法,但差异无统计学意义(t=1.227,P=0.223)。结论不同方法检测DNA倍体SPF值差异不具有统计学意义,采用单细胞制备仪方法检出非整倍体率最高,可能为最佳检测DNA倍体的方法。

Objective To research the reliability and consistency of DNA ploidy analysis by different methods. Methods The single cell preparation instrument, tissue homogenizer and paraffin-embedded tissue were respectively used to extract DNA, and DNA ploidy was detect by flow cytometry in 56 cases of esophageal cancer. Results The rates of DNA aneuploidy in 56 cases of esophageal cancer detected by the single cell preparation instrument ,tissue homogenizer and paraffin-embedded tissue were 48.21% ,44.64% and 42.86% respectively, including one was near-diploid aneuploid DNA content. The SPF value using the paraffin method was （ 18. 205 ±7. 719） % ,which was higher than the tissue homogenizer method [ （ 17. 330 ±8. 160 ） % ] and the single cell preparation instrument [ （ 15. 479 ±7.811 ） % ], but there were no statistical differences （ t = 0. 583,1. 858 ; P = 0. 561,0. 066 ） ; the SPF value got by the tissue homogenizer method was higher than by the single cell preparation instrument （t = 1. 227,P = 0. 223）. Conclusion Different methods had different SPF values, but there were no significant difference. The aneuploidy rate is highest by the detection of the single cell preparation instrument, and the method of single cell preparation instrument may be the best way.