七氟烷暴露对新生儿术后早期S100β蛋白及神经烯醇化酶表达水平的影响

Effect on the serum S100β and neuron-specific enolase levels in neonate with sevoflurane exposure during early postoperative period

目的观察七氟烷暴露对新生儿术后早期S100β蛋白及神经烯醇化酶(NSE)表达水平的影响。方法行胃肠手术新生儿60例,ASAⅠ～Ⅱ级,随机均分为3组(均n=20):呼气末七氟烷浓度(CET-SEV)×吸入七氟烷时间(Timein-hale)≦3.3%组(S1组)、3.3%<CET-SEV×Timeinhale<6.6%组(S2组)、6.6%≦CET-SEV×Timeinhale组(S3组),同期出生未接受手术的新生儿作为对照组(n=20)。实验组监测CET-SEV,于术后1 d(T1)、术后2 d(T2)、术后7 d(T3)采血,ELISA法测定血清S100β蛋白及NSE水平,对照组同期进行测试。结果与对照组比较,T1～T3时刻S1～3组S100β蛋白及S3组NSE升高差异有统计学意义(P<0.05),与T1比较,T3时刻S2组及S3组S100β蛋白和NSE降低差异有统计学意义(P<0.05)。结论七氟烷暴露诱导新生儿术后早期血清S100β蛋白和NSE水平一过性升高。

Objective To investigate serum S100β and neuron-specific enolase（NSE） levels in neonates with sevoflurane exposure during early postoperative period.Methods Sixty neonates,ASA I or II,undergoing gastrointestinal operations,were equally divided into 3 groups： endtidal concentration of sevoflurane（CET-SEV） × Time of sevoflurane inhalation（Timeinhale） ≦ 3.3% group（S1）,3.3% CET-SEV × Timeinhale 6.6% group（S2） and 6.6%≦ CET-SEV × Timeinhalegroup（S3）.Twenty neonates,borned meanwhile and undergoing sham operation,ware taken as control group（n = 20）.Trial groups were assigned to assay endtidal concentration of sevoflurane,and withdrew blood at postoperative 1 d（T1）,2 d（T2） and 7 d（T3） respectively.ELISA Kits were utilized to detect serum S100β and NSE levels,meanwhile,control group undergoing the same detections.Results Compared with control group,serum S100β in the trial groups and NSE in S3 elevated significantly at T1,T2 and T3（P 0.05）.Compared with T1,those two markers reduced significantly in S2 and S3 at T3（P 0.05）.Conclusion Sevoflurane exposure induces the transient elevation of both S100β and NSE during the early postoperative period in neonate.