贵州产春兰花香气成分分析

Study on Aromatic Components in Cymbidium goeringii in Guizhou Province

以贵州产春兰为试材,采用顶空固相微萃取(HS-SPME)和气相色谱-质谱联用(GC-MS)技术,在不同条件下对春兰花香气进行检测鉴定,以探明春兰的花香气形成的原理,促进国兰花香新品种的培育。结果表明:活体常温萃取30min和24h条件下分别都检测鉴定出了4种成分;取下花朵加热65℃萃取30min的条件下检测鉴定出14种成分。香气成分主要为烷烃类、醇类、醛类、酮酯类和烯类等。在3种情况下都检测出了β?金合欢烯、1,9-癸二炔、E,E?α?金合欢烯、橙花叔醇,其中橙花叔醇的相对含量较高。

Taking Cymbidium goeringii from Guizhou Province as test material, using the methods of headspace solid- phase microextraction（HS-SPME）and gas chromatography-mass spectroscopy（GC-MS）under different conditions, the aromatic components in Cymbidium goeringii and the principle of the formation were ascertained, the Chinese cymbidium cultivation of new varieties was promoted. The results showed that there were 4 kinds of components was identified under the different conditions（in v/vo, room temperature and extract 30 mini in vivo, room temperature and extract 24 h）, 14 kinds of components was identified under the condition（in vitro, heat and extract 30 rnin）. Including alkanes, alcohols, aldehydes, ketones, esters and terpenes etc. β-Farnesene, 1,9-Decadiyne, E. E-a-Farnesene, Nerolidol were identified under the 3 conditions. The relative content of Nerolido｜ was relatively high.