细菌分离培养与实时荧光定量-聚合酶链反应用于健康人群脑膜炎奈瑟菌携带率研究

Carriage rate of Neisseria meningitidis among healthy population with bacterium culturing and real time fluorescence quantitative polymerase chain reaction

目的了解河北省部分地区健康人群脑膜炎奈瑟菌(Nm)带菌状况,评价实时荧光定量-聚合酶链反应(real-time fluorescence quantitative Polymerase Chain Reaction,FQ-PCR)作为菌株分离培养法的补充在健康人群带菌调查中的应用价值。方法按年龄分层整群随机抽取577人采集咽拭子,采用Nm分离培养和FQ-PCR法同时检测,并使用血清学和分子生物学方法分群。结果分离到18株Nm菌株,总阳性率3.12%,其中不可分群最多(9株),其次为B群(3株)、W135群(3株)、X群(2株)、C群(1株)。FQ-PCR检测阳性样本46份,总阳性率7.97%,其中B群最多(13份),其次为W135群(12份)、不可分群(11份)、X群(7份)、C群(3份)。结论细菌培养和FQ-PCR均提示这些地区健康人群携带Nm血清群多样化。FQ-PCR检测方法在发现病原体及分群方面比传统菌株分离培养方法都有显著提升,可作为传统方法的有力补充。

Objective To understand the carrying status of Neisseria meningitidis among healthy people in selected areas of Hebei province,and evaluate the performance of real time fluorescence quantitative polymerase chain reaction（FQ-PCR） in N.meningitidis carriage detection as the supplement to conventional culturing.Methods Throat swabs were taken from 577 healthy people selected by stratified cluster random sampling based on age,and the isolation of N.meningitidis was conducted with both culturing and FQ-PCR.Results Totally 18 strains of N.meningitidis were isolated from the throat swabs（3.12%）,in which 9 were unspecified strains,3 were serogroup B strains,3 were W135 strains,2 were X strains and 1 was C strain.Totally 46 samples were detected to be positive by FQ-PCR（7.97%）.The most common serogroup was B（13）,followed by W135（12）,unspecified（11）,X（7） and C（3）.ConclusionVariety of N.meningitidis serogroups were detected among healthy carriers in surveyed areas with both culturing and FQ-PCR,but the detection rate of FQ-PCR was significantly higher than that of conventional bacterium culturing.