银胡感冒海绵剂的体外透皮试验

In vitro Transdermal Test of Yinhu Ganmao Spongia

目的:考察银胡感冒海绵剂的体外透皮吸收性能。方法:采用Franz扩散池法进行体外透皮扩散试验,以10%乙醇为接收液,收集不同时间点的透皮接收液,通过HPLC测定绿原酸含量,GC测定芳樟醇含量,计算指标成分的累积渗透量和渗透速率。结果:绿原酸、芳樟醇的累积渗透量回归方程分别为Q=7.143 8t+8.229 4(r=0.996 1),Q=125.375t-143.705(r=0.989 8),透皮速率依次为7.144,125.375μg.cm-2.h-1。结论:银胡感冒海绵剂中绿原酸在24 h内持续均匀渗透,而芳樟醇基本在12 h大部分已渗透;该方法简便,快速,是综合评价银胡感冒海绵剂体外透皮性能的理想方法。

Objective： To investigate in vitro transdermal absorption properties of Yinhu Ganmao spongia. Method： The content of chlorogenic acid was determined by HPLC and GC determination of linalool content, release was detected by Franz diffusion cell method with 10% ethanol as receiving liquid, transdermal receiving liquid at different time points was collected, and calculated cumulative permeation amount and ratio. Result： Regression equations of cumulative permeation amount of chlorogenic acid and linalool were Q = 7. 143 8t＋8.229 4 （r =0.996 1）, Q = 125.375t-143.705 （r =0.989 8）, respectively. J of them were 7. 144, 125.375 μg · cm-2 · h^-1, respectively. Conclusion： Chlorogenic acid in Yinhu Ganmao spongia continuous permeated uniformly within 24 h, but linalool almost permeated compeletly in 12 h; This method was simple and rapid, it was a ideal method to comprehensive evaluate in vitro transdermal performances of Yinhu Ganmao spongia.