天山花楸枝叶中总黄酮、氰苷的大孔吸附树脂纯化工艺优选

Optimization of Purification Technology for Total Flavonoids and Cyanogenetic Glycosides in Leaves of Sorbus tianschanica by Macroporous Resin

目的:优选大孔吸附树脂纯化天山花楸枝叶中总黄酮、氰苷类成分的工艺条件。方法:以比吸附量、吸附率、洗脱率为指标,采用静态吸附试验筛选大孔树脂,单因素试验考察上样液质量浓度、吸附流速、树脂柱径高比、洗脱剂浓度、洗脱流速等工艺参数对纯化工艺的影响。结果:采用HPD-100型大孔吸附树脂,优选的纯化工艺为上样液质量浓度0.1 g.mL-1,最大上样量2.2 g生药/g干树脂,径高比1∶6,吸附流速2 BV.h-1,上样后静置30 min,加50%乙醇4 BV以2 BV.h-1流速,收集洗脱液。结论:HPD-100型大孔树脂对天山花楸枝叶中总黄酮、氰苷纯化效果较好,乙醇洗脱物中有效部位质量分数>50%。

Objective： To optimize purification technology of total flavonaoids and cyanogentic glycosides in leaves of Sorbus tianschanica by maeroporous resin. Method： With absorption capaeity, absorption rate and desorption rate as indexes, 5 different macroporous resins were screened by static adsorption test, effects of the concentration of sample solution, ratio of diameter to height, adsorption velocity, the concentration of ethanol, elution velocity and other process parameters on purification technology of total flavonoids and cyanogenetic glycosides in leaves of S. tianschanica were investigated by single factor tests. Result： HPD-100 resin was adopted, its optimum purification technology was as following： absorption capacities of crude drugs to resin 2.2 g ·g^-1 ,the concentration of sample solution 0. 1 g ·mL^-1 with ratio of diameter to height at 1 ： 6, adsorption velocity at 2 BV ·h^-1, standing for 30 rain, eluted with 4 BV 50% ethanol, elution velocity 2 BV .h -t , collected eluent. Conclusion： HPD-100 resin could be used successfully to purify total flavonaoids and cyanogentic glycosides in leaves of S. tianschanica with the mass fraction of effective part in ethanol eluate was more than 50%.