比色法测定龙血竭总黄酮含量

Content Determination of Total Flavonoids in Resina Draconis by Colorimetric Method

目的:对龙血竭总黄酮的比色测定方法进行研究。方法:利用龙血竭总黄酮可与各种不同的显色试剂作用并产生颜色的特性,比较显色后的吸收曲线变化差异。结果:龙血竭用HCl-Mg粉显色后在475 nm处产生一个大的吸收峰,且此处的干扰较小,在6 h内稳定性良好,在0.012～0.12 g.L-1呈良好的线性关系(r=0.999 3);用其他显色剂显色后吸收峰均无太大变化。结论:总黄酮测定常用的AlCl3,NaNO2-Al(NO3)3-NaOH比色法不适合龙血竭总黄酮的测定,HCl-Mg粉可作比色法测定龙血竭总黄酮的显色剂,但仍需寻找合适的对照品。

Objective： To study the colorimetric determination of total flavonoids in Resina Draconis. Method： Comparison on the difference of absorption curve was done using the characteristics that total flavonoids of Resina Draconis can produce color with various chromogenic reagents. Result： Resina Draconis colored by HCl- Mg power produce a large absorption peak at 475 nm where the interference is small. It had good stability within 6 h and showed a good linear relationship in the range of 0. 012-0. 12 g ·L^-1 （r = 0. 999 3 ）. And its absorption peak was not much change after colored by other chromogenic agent. Conclusion： A1Cl3, NaNO2-Al （NO3）3-NaOH colorimetries usually used for determination of total flavonoids were not suitable for Resina Draconis flavones assay. HCI-Mg powder can be used as chromogenic agent for colorimetric determination of the total ＂flavonoids in Resina draconis. But it still needs to find a suitable reference substance.