HPLC同时分析山蜡梅叶中4种有效成分的含量

Simultaneous Determination of Four Active Compounds in Chimonanthus nitens by HPLC

目的:建立HPLC同时测定山蜡梅叶中4种有效成分的含量。方法:采用依利特hypersil ODS2(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速1.0 mL.min-1,检测波长344 nm,柱温40℃。结果:东莨菪内酯在5.665～56.65 mg.L-1线性关系良好(r=0.999 9),平均回收率为97.06%,RSD 1.62%(n=6),异嗪皮啶在2.431 2～24.312 mg.L-1线性关系良好(r=0.999 9),平均回收率为102.86%,RSD 0.93%(n=6),滨蒿内酯在2.444～24.44 mg.L-1线性关系良好(r=0.999 9),平均回收率为102.18%,RSD 1.81%(n=6),芦丁在9.012～90.12 mg.L-1线性关系良好(r=0.999 2),平均回收率为104.44%,RSD 4.2%(n=6)。结论:方法操作简便,结果准确,重复性好,可用来控制山蜡梅药材的质量。

Objective： To set up the method for simultaneous determination of four active compounds in Chimonanthus nitens by HPLC. Method： The chromatography separation was performed on the Elite hypersil ODS2 （4.6 mm × 250 mm, 5 μm） with gradient elution. The mixture of acetonitrile and water as the mobile phases at a flow rate of 1.0 mL ·min^-1 was used, the detective wavelength was set at 344 nm and the column temperature was kept at 40 ℃. Result： The calibration curve was linear in the range of 5. 665-56.65 mg .L^-1 for scopoletin （ r = 0. 999 9） , the average recovery was 97.06% and RSD was 1.62%. The calibration curve was linear in the range of 2. 431 2-24. 312 mg ·L^-1 for isofraxidin （r =0. 999 9）, the average recovery was 102.86% and RSD was 0.93%. The standard curve presented a linear range from 2.444-24.44 mg · L^-1 for scoparone （r =0. 999 9） ,the average recovery was 102. 18% and RSD was 1.81%. The standard curve presented a linear range from 9. 012-90. 12 mg·L^-1 for rutin （r = 0. 999 2）, the average recovery was 104. 44% and RSD was 4.2%. Conclusion： The method is available with a good reproducibility, sensitive and simple and could be used to control the quality of C. nitens.