HPLC测定复方贯叶金丝桃滴丸中金丝桃苷的含量

HPLC Determination of Hyperin in Compound Hypericum Perforatum Dropping Pills

目的:建立复方贯叶金丝桃滴丸中主要有效成分金丝桃苷的含量测定方法。方法:采用高效液相色谱法,COSMOSIL packed Column 5 C18-MS-Ⅱ(4.6 mm×250 mm,5μm)色谱柱,流动相乙腈-水(0.5%冰醋酸和2%四氢呋喃)(13∶87),检测波长360 nm。结果:金丝桃苷在10.8～54.0 mg.L-1峰面积与进样浓度具有良好线性关系(r=0.999 1),平均回收率98.31%(n=6),RSD 1.23%。结论:该法准确、可行,可用于该滴丸的质量控制。

Objective： To establish the method to determine the concentration of hyperin in Compound Hypericum Perforatum Dropping Pills. Method： HPLC was used for the determination of hyperin in Compound Hypericum Perforatum. A COSMOSIL packed column 5 C18-MS- 11 （4. 6 mm × 250 mm, 5 μm） was used. The mobile phase was acetonitrile-water （0.5% glacial acetic acid and 2% tetrahydrofuran） （13：87）. The detection wavelength was set at 360 nm. Result： The linear range for hyperin was 10. 8-54.0 mg ·L^-1 （r =0. 999 1 ）. The average recovery was 98.31% （RSD 1.23% ）. Conclusion： The method can control the quality of Compound Hypericum Perforatum Dropping Pills effectively. It was sensitive, accurate and feasible.