银杏内酯B的傅里叶变换红外光谱-拉曼光谱分析

FTIR-Raman Analysis of Ginkgolide B

目的:建立银杏内酯B的傅里叶变换红外光谱-拉曼光谱(FTIR-Raman)联用分析方法。方法:利用脂肪提取器对银杏叶中银杏内酯B进行提取、柱层析法纯化、烘干后进行红外光谱和拉曼光谱测定,与银杏内酯B对照品经同法结晶后测得的谱图进行比较分析。结果:样品谱图与对照品谱图特征峰峰形、峰位一致,IR谱图与Raman谱图相互补充,数据完整。结论:傅里叶变换红外光谱-拉曼光谱联用分析方法准确可靠,可用于物质的结构鉴定。

Objective： To establish a FTIR-Raman analytic method of the ginkgolide B. Method： The ginkgolide B was extracted by fat extractor, and was purified by column chromatography, and was tested by the IR and Raman spectrometry after drying, and the spectrograms were compared with the FTIR spectrogram and Raman spectrogram of ginkgolide B reference substance which was treated with processing method of the sample. Result： The peak shape and peak position were consistent with the sample spectrogram and reference spectrogram, the IR spectrogram and Raman spectrogram can complement each other, so data are complete. Conclusion： The method is accurate and reliable, it can be used for the identification of substances.