天舒胶囊对利血平致小鼠偏头痛的影响

Effect of Tianshu Capsule on Migarine Model Induced by Reserpine

目的:观察天舒胶囊对利血平致小鼠偏头痛模型的作用,并探讨其作用机制。方法:ICR小鼠背部皮下注射利血平0.1 mg.kg-1造模,共12 d,正常对照组注射0.9%氯化钠注射液,于造模第7天开始ig给予天舒胶囊,剂量分别为生药1.55,4.65 g.kg-1,连续6 d,第12天测定小鼠痛阈值,次日摘眼球采血检测凝血时间,取脑部组织分别测定5羟色胺(5-HT)、去甲肾上腺素(NA)含量,阳性对照为佐米曲普坦片(0.66 mg.kg-1)。结果:与模型组相比,天舒胶囊能明显提高小鼠痛阈值(P<0.01),延长凝血时间(P<0.01),提高脑部NA和5-HT含量(P<0.05,P<0.01),与阳性药相比,天舒胶囊明显提高脑部5-HT的含量(P<0.05)。结论:天舒胶囊对利血平低化5-HT致小鼠偏头痛有明显的改善作用,其平衡神经递质释放、抑制偏头痛发作期血管过度扩张可能是作用机制之一。

Objective： To study the effect and mechanism of Tianshu capsule on the migraine＇s mice, which was composed of Gastrodiae Rhizoma and Ligustici Chuanxiong Rhizoma. Method： Model was building on ICR mice after subcutaneous injection of reserpine 0. 1 mg·kg^-1·d^-1× 12 d, control group mice were injected 0.9% sodium chloride injection, treatment were started on the seventh day of modeling for 6 consecutive days, dose were 1.55, 4.65 g·kg^-1. The pain threshold was tested on the twelve day, the clotting time was tested next day and concentration of serotonin （5-HT）, noradrenaline （NA） in the brain, was detected. Zolmitriptan tablets were used as a positive durg. Result： Compared with model group, the tianshu capsule could increase the pain threshold （P 〈 0.01 ）, prolong the clotting time （P 〈 0.01 ）, increase the concentration of 5-HT and NA in brain （P 〈 0.05, P 〈 0.01 ）. Compared with positive drug, the capsule could increase the concentration of 5-HT （P 〈 0.05）. Conclusion： The capsule can treat the migraine＇s mice by balancing release of 5-HT, inhibiting vascular dilatation of migraine attacks.