四君子汤传统煎煮和机器煎煮的药效学差异比较

Pharmacological Comparison between Traditional Decoction and Machine Decoction of Sijunzi Tang

目的:对3种煎煮工艺所得的四君子汤进行药效学评价。方法:采用大黄造成的大鼠脾虚模型,随机分为模型组,空白对照组,传统煎煮汤剂、常压煎煮汤剂、高压煎煮汤剂10,5,2.5 g.kg-1组,西沙必利阳性对照组(0.008 g.kg-1)。采用利血平腹腔注射造成小鼠脾虚模型,随机分为模型组,空白对照组,传统煎煮汤剂、常压煎煮汤剂、高压煎煮汤剂(13.2,6.6,3.3 g.kg-1),香砂养胃丸阳性对照组(2 g.kg-1),以一般体征、血清胃泌素、脏器指数、唾液淀粉酶、D-木糖排泄率为指标,比较3种煎煮工艺的药效学差别。结果:2种脾虚动物经过四君子汤治疗后,其脾虚症状得到显著改善,3种煎煮工艺所得四君子汤均能显著改善脾虚动物的脾虚症状,指标结果显示传统煎煮稍优于机器煎煮,但3种煎煮工艺的不同的四君子汤对脾虚动物的治疗作用无显著性差异。结论:3种煎煮工艺所得四君子汤对脾虚动物的治疗作用无显著性差异。

Objective： To compare the pharmacological effect of three Sijunzi Tang adopted different processing technology. Method： The pharmacodynamic differences for three decoctions of Sijunzi Tang processed by different technics were compared, using two kinds of spleen-deficiency model. One model was induced by Pendulifory Elshortzia. The rats were randomly divided into the model group, the blank control group, Sijunzi Tang groups with high, medium and low doses （10, 5, 2.5 g·kg^-1） , Sijunzi Tang adopted the high pressure processing with high, medium and low doses （10, 5, 2.5 g·kg^-1）, Sijunzi Tang adopted the atmospheric pressure processing with high, medium and low doses （10, 5, 2.5g·kg^-1）, and eisapride as the positive control group. The other model was induced by reserpin. The mice were randomly divided into the model group, the blank control group, Sijunzi Tang adopted the traditional decoction processing with high, medium and low doses （13.2, 6.6,3.3 g·kg^-1 ） , Sijunzi Tangadopted the high pressure processing with high, medium and low doses （ 13.2, 6.6, 3.3 g·kg^-1 ） , Sijunzi Tang adopted the atmospheric pressure processing with high, medium and low doses （ 13.2, 6.6, 3.3 g·kg^-1）. Xiangsha Yangwei pills were used as positive control. The criteria included general physical signs, D-xylose excretion rate, amylase activity, serum gastrin content and indexes of organs determined before and after treatment. Result： After treatment with Sijunzi Tang, the spleen symptoms of spleen-deficiency animals were improved, and the final data analysis showed that Sijunzi Tang could significantly improve spleen-deficiency symptoms. The indicators showed that Sijunzi Tang adopted the traditional processing was slightly better than that adopted the machine processing. However, there was no significant difference among the three Sijunzi Tang adopted different processing technics for the therapeutic effect on the spleen-deficiency animals. Conclusion： The three processing technics of Sijunzi Tang show similar therapeutic effect for the spleen-deficiency animals.