摘要
目的 评价非小细胞肺癌裸鼠体内肿瘤药物筛选结果及临床应用疗效。方法 新鲜非小细胞肺癌组织块植入裸鼠肾包膜下 ,选用常用抗非小细胞肺癌药物进行肿瘤药敏试验。临床实验组依据肿瘤药敏结果进行肺癌根治术后辅助化学治疗 ;临床对照组选取同期非小细胞肺癌根治术后接受CAP方案辅助化学治疗。结果 人体癌细胞植入裸鼠体内后能很好地保持肿瘤细胞形态 ,移植瘤细胞可见率为 87 8%。临床实验组 3a生存率为 60 0 % ,临床对照组 3a生存率为 5 0 0 % ,差异无显著性 (P >0 0 5 ) ,两组Ⅲ期 3a生存率分别为 5 2 6%和 3 3 3 % ,差异有显著性 (P <0 0 5 )。结论 裸鼠肾包膜下筛选抗肿瘤药物进行术后辅助化疗有非常显著临床效果 ,3a生存率有一定提高 ,避免肿瘤化学治疗上的盲目性。
Objective To evaluate chemosensitivity of non-small cell lung cancer(NSCLC) in nude mice subrenal capsule assay and efficacy of adjuvant chemotherapy after radical surgery for NSCLC. Methods\ Fresh non-small lung cancer specimens were transplanted into nude mice and 10 kinds of potential anticancer drugs were used in vivo chemosensitivity test. 120 patients with NSCLC undergone radical surgery were randomized into two groups: 70 patients received adjuvant chemotherapy based on chemosensitivity testing in nude mice assay, 50 patients received adjuvant chemotherapy with standard medicines. Results\ The histological examination showed that many well preserved tumor cells in nude mice on day 12 and individual lung cancers showed different sensitivities to the drugs. The 3-year survival rate was 60.0% in the nude mice assay group , and 50.0% in the contral group. The difference between two groups was not statistical significant( P >0 05). But in stage Ⅲ, the survival rate was 52 6% and 33 3%, with statistically significance( P <0 05). Conclusion\ The tumor chemosensitivity testing in nude mice assay showed an excellent clinic results, Postoperative adjuvant chemotherapy based on chemosensitivity testing in nude mice assay results in significantly higher 3-year survival rate in stage III than in standard medicines.
出处
《广东医学》
CAS
CSCD
2000年第9期728-730,共3页
Guangdong Medical Journal
基金
广州市科委重点科技攻关项目 !(编号 :98-Z - 0 2 3- 0 1 )
关键词
裸鼠
药物筛选
辅助化疗
肺小细胞肺癌
Nude mice Tumor chemosensitivity test Postoperative adjuvant chemotherapy