酶解小黑豆乳清多肽抗肿瘤作用的研究

Study on anti-tumor activity of enzymolysis whey peptides from small black soybean

研究以小黑豆乳清为原料,比较木瓜蛋白酶、537酸性蛋白酶、ProteAX蛋白酶三种蛋白酶水解多肽产物的分子量分布及其对小鼠H22肿瘤细胞生长抑制作用。结果表明,ProteAX酶解多肽的分子量分布明显不同于另外两种多肽,且对小鼠H22肿瘤细胞生长抑制率最高,当ProteAX酶解多肽浓度为40mg/mL时,可达51.36%。用ProteAX酶解多肽喂食用氨基比林-亚硝酸钠(AP-NaNO2)诱导的肝损伤模型小鼠,结果显示,与模型组相比,该多肽可以显著降低肝脏MDA和血清ALT、AST活性(p<0.05),并提高肝脏GSH-Px活性(p<0.05)。肝脏组织切片结果显示,模型组小鼠大部分肝细胞形态不正常,细胞损伤严重,出现明显的癌前期病变;而小黑豆乳清多肽高剂量组动物的肝细胞形态基本正常,接近正常肝组织;提示ProteAX蛋白酶解多肽可以抑制AP-NaNO2诱导的肝细胞损伤和癌前期病变。

Molecular weight and antitumor effect in vitro of small black bean whey peptides were studied. Whey protein was hydrolyzed by papain,537 acid proteinase and Prote AX protease separately. Results indicated that molecular weight distribution of Prote AX enzymolysis polypeptide was obviously different from the other two polypeptides. It indicated that H 22 tumor cell growth repression rate of Prote AX enzymolysis polypeptide was the highest,which reached to 51.36% when the peptide concentration was 40mg/mL. Mice were fed Prote AX enzymolysis polypeptide in AP-NaNO 2 -induced liver injury model. Results showed that MDA,ALT,AST and GSH-Px of mice in high dose group were significantly higher than those of mice in the model group（p＆lt;0.05）. Results of histology slice examination showed that liver cells in the model group were abnormal and injuerd seriously,pre-cancerous lesions were appeared. However,the liver cells of mice in high Prote AX enzymolysis polypeptide dose group were close to normal liver tissue. Therefore,the AP -NaNO 2 -induced liver injury and pre-cancerous lesions could be inhibited by Prote AX enzymolysis polypeptide.