他莫昔芬和甲孕酮对人卵巢癌细胞系增殖和凋亡的影响

Effects on the proliferation and apoptosis of human ovarian cancer cell line treated with tomoxifen and medroxyprogesterone

研究他莫昔芬和甲孕酮对人卵巢癌细胞增殖和凋亡的影响。方法 :用不同剂量的他莫昔芬和甲孕酮与人卵巢癌细胞系HO 891 0体外培养 96h ,用苔盼蓝活细胞拒染法计活细胞数 ,免疫组化SABC法检测癌细胞增殖核抗原 (PCNA)表达情况 ,DNA缺口原位末端标记方法 (TUNEL)检测细胞凋亡情况。结果 :他莫昔芬和甲孕酮在 0 1、1、1 0 μmol均可使HO 891 0活细胞数显著减少 (P <0 0 1 ) ;通过诱导细胞凋亡抑制卵巢癌细胞生长 ,呈剂量依赖性。低浓度 (≤ 1 μmol L)的他莫昔芬对PCNA表达的影响无统计学意义 (P >0 0 5 ) ,而高剂量 ( 1 0 μmol L)时可显著降低PCNA表达 (P <0 0 5 )。甲孕酮在 0 1、1、1 0 μmol时均明显降低PCNA表达 (P <0 0 1 ) ;他莫昔芬与甲孕酮均可诱导HO 891 0细胞凋亡 (P <0 0 1 ) ,且呈剂量依赖性 ,甲孕酮诱导凋亡程度显著高于他莫昔芬 (P <0 0 1 )。结论 :他莫昔芬在低剂量时对细胞增殖和抑制作用较弱 ,高剂量时不但可抑制卵巢癌细胞增殖 ,且可诱导细胞凋亡 ,但均弱于甲孕酮 。

Purpose:To investigate the effects of tomoxifen(TAM) and medroxyprogesterone(MPA) on the proliferation and apoptosis on human ovarian cancer cell.Methods:Human ovarian cancer cell line HO 8910 was cultued with different doses TAM and MPA. 96 h later, the number of HO 8910 viable cells was counted by trypan blue exclusion assay. The expression of proliferation cell nuclear antigen (PCNA) in HO 8910 was detected with immunohistochemical stining (SABC), and HO 8910 cell apoptotic index (AI) was detected by DNA in situ terminal deoxynucleotidyl transferase mediated dUTP biotin nick ending labeling (TUNEL). Results:The viable carcinoa cells were significantly reduced in dose dependent fashion after treatment 96 h with TAM and MPA(P<0 01).At low doses (≤1 μmol/L),TAM did not reduce the PCNA expression of HO 8910( P >0 05),wheras at high dose (10 μmol/L) these resulted in signicant reduction of PCNA expression (P<0 01). At different doses (0.1, 1, 10 umol/L),MPA could decrease PCNA expression. Both TAM and MPA could induce apoptosis of HO 8910 (P<0 01) Conclusions:The antitumor effect of TAM and MPA is related to their dosage. TAM could inhibite the proliferation and induce apoptosis of ovarian carcinoma cell, but weaker than MPA. The results provide some clues for the endocrinal therapy of ovarian carcinoma.