水蒸汽蒸馏法与超临界二氧化碳萃取法提取山桔叶挥发油成分分析

Extraction of the volatile compounds from Folium glycosmis parviflorae by steam distillation and supercritical fluid extraction

目的:分析比较水蒸汽蒸馏提取法与超临界CO2萃取法提取山桔叶挥发油差异。方法:采用水蒸汽蒸馏法(SD)及超临界CO2萃取法(SFE)提取山桔叶挥发油,并用气相色谱-质谱联用法分析鉴定其化学成分。结果:SD法提取的挥发油分离出60个色谱峰,共鉴定38个化合物,占挥发油总量的92.28%;SFE提取的挥发油法分离出59个色谱峰,鉴定27个化合物,占挥发油总量的72.13%。结论:2种提取方法所得的化学成分组成和含量有较大差异。

OBJECTIVE To analyze the chemical constituents of the volatile compounds from Folium glycosmis parviflorae by different extraction methods.METHODS The volatile compounds were extracted by steam distillation（SD） and by supercritical fluid extraction（SFE） separately,constituents were separated and identified by GC-MS.RESULTS Sixty compounds were isolated and thirty-eight compounds were identified by SD which composed about 92.28% of the total essential oils;and fifty-nine compounds were isolated and twenty-seven compounds were identified by SFE that composed about 72.13% of the total essential oils.CONCLUSION There are differences in the chemical constituents and contents by the two extraction methods.