见血青提取物止血作用的实验研究

Study on the Hemostatic Effect of the Extracts from Liparis nervosa

目的:研究见血青的止血、促凝血作用。方法:小鼠分别灌胃高、低剂量(4、2g/kg)见血青水提取物、石油醚提取物、二氯甲烷提取物、水醇液提取物,每天1次,连续7d后剪尾测定止血时间(BT);毛细管法测定凝血时间(CT)。大鼠分别灌胃高、低剂量(4、2g/kg)见血青水提取物、石油醚提取物、二氯甲烷提取物、水醇液提取物,每天1次,连续7d后颈总动脉采血,测定凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(FLB)含量。结果:高、低剂量见血青二氯甲烷提取物可显著缩短小鼠BT(P<0.05),低剂量见血青二氯甲烷提取物可显著缩短小鼠CT和大鼠APTT(P<0.05),高、低剂量见血青水提取物与石油醚提取物、二氯甲烷提取物、水醇液提取物可显著增加大鼠FIB含量(P<0.05)。结论:见血青具有较好的止血、促凝血作用,推测其作用机制可能与收缩血管、抑制纤溶酶活性、增加FIB含量有关。

OBJECTIVE： To study the effect of Liparis nervosa on promoting hemostasis and blood coagulation. METHODS： The mice were given high-dose and low-dose（4,2 g/kg） aqueous extract, aether petrolei extract, dichloromethane extract, water ex- traction and alcohol precipitation of L. nervosa intragastrically once a day for consecutive 7 days. The bleeding time （BT） was de- termined by shearing tail method. Clotting time（CT） was detemined by capillary tube method. The rats were given high-dose and low-dose（4,2 g/kg） aqueous extract, aether petrolei extract, dichloromethane extract, water extraction and alcohol precipitation of L. nervosa intragastrically once a day for consecutive 7 days. The blood samples were collected from carotid artery for the determi- nation of prothrombin time （PT）, activated partial thromboplastin time （APTT）, thrombin time （TT） and fibrinogen （FIB）. RESUL- TS： High-dose and low-dose dichloromethane extract of L. nervosa could shorten BT of mice significantly（P〈0.05） ; CT and the APTT values were obviously shortened by low-dose dichloromethane extract（P〈0.05）, and FIB values were obviously increased by all of the extracts of L. nervosa（P〈0.05）. CONCLUSIONS ：L. nervosa has an effect on promoting hemostasis and blood coagula- tion, and its mechanisms may be related with shrinking blood vessels, inhibiting plasmin activity and rising FIB content.