醒神喷鼻液对出血模型动物的保护作用研究

Study on the Protective Effect of Xingshen Nasal Spray on Cerebral Hemorrhage Model Animal

目的:研究醒神喷鼻液对出血模型动物的保护作用。方法:参考文献复制大鼠脑出血模型,以神经症状评分判断模型是否复制成功。60只大鼠分为正常对照(滴鼻,空白基质)组、模型(滴鼻,空白基质)组、醒脑静(腹腔注射,7.6ml/kg)组与醒神喷鼻液高、中、低剂量(滴鼻,0.496、0.248、0.124g/kg)组。大鼠给药3d后复制模型,复制模型成功后,大鼠再给药1d,末次给药24h后取血液和脑组织,酶联免疫法测定内皮素(ET)、降钙素基因相关肽(CGRP),计算ET/GRP值。50只小鼠分为正常对照(滴鼻,空白基质)组、氨甲苯酸(腹腔注射,2.4mg/kg)组与醒神喷鼻液高、中、低剂量(滴鼻,1.08、0.54、0.27g/kg)组,每天给药2次,连续4d,末次给药30min后,剪尾法测定出血时间。结果:与模型组比较,醒神喷鼻液高剂量组大鼠脑组织中CGRP含量显著增加(P<0.05),醒神喷鼻液高、中、低剂量组大鼠血液中的CGRP含量显著增加,脑组织与血液的ET/CGRP显著降低(P<0.01或P<0.05)。与正常对照组比较,醒神喷鼻液高、中、低剂量组小鼠出血时间显著缩短(P<0.01或P<0.05)。结论:醒神喷鼻液能减轻模型动物脑组织损伤程度和保护脑神经细胞,可减少出血量,减轻出血引起的脑水肿程度。

OBJECTIVE： To study the protective effect of Xingshen nasal spray on cerebral hemorrhage model animal. METH- ODS： By literature review, the cerebral hemorrhage model of rat was induced. The neurological symptom score was applied to eval- ute the models. Sixty rats were divided into normal control group （nasal drops,matrix）,model group（matrix, nasal drops）, Xing- naojing group （7.6 ml/kg, intraperitoneal injection） and Xingshen nasal spray high-dose, medium-dose and low-dose groups （0.496, 0.248, 0.124 g/kg, nasal drops）. The cerebral hemorrhage model of rats was induced 3d after medication. After modeling, model rats were given medicines for a day, and blood and cerebal samples were collected 24h after last administration. The levels of ET and CGRP were determined by ELISA, and ET/CTRP was calculated. Fifty mice were divided into normal control group （ma- trix, nasaldrops）, aminomethylbenzoic acid group （2.4 mg/kg, intraperitoneal injection） and Xingshen nasal spray high-dose, medi- um-dose and low-dose groups （1.08, 0.54, 0.27 g/kg, nasal drops）. They were given medicines twice a day for consecutive 4 d. 30 rain after the last administration, bleeding time was determined with the method of tail cuting. RESULTS： Compared with model group, the levels of CGRP in blood tissue increased significantly in Xingshen nasal spray high-dose group（P〈0.05）. The levels of CGRP in blood increased significantly in Xingshen nasal spray high-dose, mediume-dose and low-dose groups, while ET/CGRP de- creased in cerebral tissue and blood. Compared with normal control group, the bleeding time of rats in Xingshen nasal spray high-dose, mediume-dose and low-dose groups could be shortened （P〈 0.01 or P〈 0.05）. CONCLUSIONS： Xingshen nasal spray can relieve cerebral injury and protect cerebral nerve cells of the model animal, and can decrease the amount of bleeding and the levels of brain edema caused by bleeding.