当归芍药散含药血清对大鼠心肌细胞损伤的保护作用研究

Protective Effects of the Medicinal Serum of Danggui Saoyao Powder on Myocardial Cells Injury of Rats

目的:研究当归芍药散含药血清对大鼠心肌细胞损伤的保护作用。方法:于缺氧环境下培养SD乳鼠原代心肌细胞4h后在含氧环境下继续培养以复制心肌细胞缺氧再灌注模型。试验分为空白对照组、利多卡因含药血清组与当归芍药散高、低剂量含药血清组。分别于缺氧前,缺氧60、120、180min时间点与缺氧120min后再给氧30、60min时间点采集标本检测培养液中的肌酸磷酸激酶(CPK)、碱性磷酸酶(ALP)、天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、血管紧张素转换酶(ACE)的活性。结果:与空白对照组比较,当归芍药散高、低剂量含药血清组在缺氧前,缺氧60、120、180min时间点与缺氧120min后再给氧30、60min时间点心肌细胞内CPK、ALP活性显著减弱(P<0.01);当归芍药散高、低剂量含药血清组在缺氧120、180min时间点与缺氧120min后再给氧30、60min时间点ACE活性显著减弱(P<0.01);当归芍药散高剂量含药血清组在缺氧120、180min时间点与缺氧120min后再给氧30、60min时间点AST、LDH活性显著减弱(P<0.01)。结论:当归芍药散含药血清可减轻心肌细胞缺氧再灌注损伤,对心肌细胞具有一定保护作用。

OBJECTIVE： To study the protective effects of the medicinal serum of Danggui saoyao powder on myocardial cells injury of rats. METHODS： Primary cultured ventricular myocytes of SD neonatal rat were cultured under oxygen-deficient environ- ment, and 4 h later cultured under aerobic environment to establish myocardial anoxia-reperfusion model. Model rats were divided into blank control group, lidocaine group and Danggui saoyao powder high-dose and low-dose medicinal serum groups. The sam- pies were collected before hypoxia, at 60 min, 120 min and 180min after anoxia, at 30 and 60 min after reoxygenation following 120 min anoxia. The changes of CPK, ALP, AST, LDH and ACE activity from the culture solution were determined, the effects of the medicinal serum of Danggui saoyao powder on these indicators were also observed. RESULTS： Compared with blank control group, the activities of CPK and ALP in myocardial cells decreased significantly in medicinal serum high-dose and low-dose groups before hypoxia, at 60 min, 120 min and 180 min after anoxia, at 30 and 60 min after reoxygenation following 120 min anoxia （P〈0.01）.While the activities of ACE decreased significantly at 120 min and 180 min after anoxia, at 30 and 60 min after reoxy- genation following 120 min anoxia（P〈0.01 ）. The activities of AST and LDH in myocardial cells decreased significantly in medici- nal serum high-dose groups before hypoxia, at 60 min, 120 min and 180min after anoxia, at 30 and 60 min after reoxygenation fol- lowing 120 min anoxia（P〈0.01）.CONCLUSIONS： The medicinal serum of Danggui saoyao powder can reduce the myocardial an- oxia-reperfusion injury and display protective effect on myocardial cells directly.