陈皮中橙皮苷的近红外光谱定量模型的建立

Quantitative Model for Hesperidin Content in Citrus reticulate by NIR Spectroscopy

目的:建立陈皮中橙皮苷的近红外光谱定量模型,快速测定陈皮中橙皮苷的含量。方法:采用高效液相色谱法测定213批陈皮中橙皮苷的含量;采集所有样品的近红外光谱,并用一阶导数预处理,结合偏最小二乘法建立橙皮苷的定量模型。结果:校正模型的内部交叉验证决定系数(R2)达到0.93198,校正均方差为1.00985,预测均方差为0.948,主成分数为9;44批验证集样品的平均预测回收率为101.08%。结论:所建立的近红外光谱定量模型稳定、准确、可靠,可快速测定陈皮中橙皮苷的含量。

OBJECTIVE： To develop NIR quantitative analysis model of hesperidin and a method for the determination of hes- peridin in Citrus reticulate. METHODS： The content of in 213 batches of C. reticulate were determined by HPLC. NIR spectro- grams were measured, and the first derivative of Savitsky-Golay was used as spectral preprocessing options, and calibration model of hesperidin was established by the partial least squares regression analysis. RESULTS： Correlation coefficients （R2）, root-mean-square error of calibration and root-mean-square error of prediction for hesperidin were 0.931 98, 1.009 85,0.948. There were 9 principal components and average recoveries of 44 batches of validation set were 101.08%. CONCLUSIONS：The method is steady, accurate and reliable, and can be used for the content determination of hesperidin in C. reticulate.