高效嗜碱好氧反硝化细菌的筛选及其脱硝特性

Isolation and Nitrogen Removal Characteristics of an Efficient Alkalophilic Aerobic Strain Denitrifier

从碱厂污泥中筛选出一株高效嗜碱好氧反硝化细菌BMEN1,通过生理生化鉴定和16S rDNA序列分析,鉴定为Pseudomonasstutzeri,命名为Pseudomonas stutzeriBMEN1,其具有napA,nirK,norB和nosZ等好氧反硝化相关基因.通过条件实验确定该菌最适脱硝条件为:pH 9.0,37℃,有效碳源依次为丁二酸钠、乙酸钠和葡萄糖等.在最适条件下,以菌体浓度计脱硝(NO_3^--N)速率高达52.92 mg/(h.OD),且无NO_2^-累积.将其用于模拟烟道气吸收液脱硝,能在16 h内完全脱除NO_3^--N和NO_2^--N 140 mg/L,表明该菌是有应用潜力的好氧反硝化脱硝细菌.

An alkalophilic aerobic denitrification strain BMEN1 was screened from alkali plant sludge. According to the biochemical and physiological characteristics and sequence analysis of 16S rRNA gene, strain BMEN1 was identified as Pseudomonas stutzeri and named as P stutzeri BMEN1. The aerobic denitrification-relevant genes napA, nirK, norB and nosZ were noted in P stutzeri BMEN1. The optimal temperature and pH value for aerobic denitrification were 37 ＊C and 9.0, and the available carbon sources were sodium succinate, sodium acetate and glucose. Under the optimum conditions, nitrogen （NO3--N） removal rate ofP. stutzeri BMEN1 reached up to 52.92 mg/（h.OD）, and in the experiment of synthetic absorbed solution of flue gas, it could quickly remove away about NO3--N 140 mg/L and NO2--N 140 mg/L within 16 h. These results suggested that P. stutzeri BMEN1 was a potential efficient aerobic strain denitrifier.