慢性乙型肝炎病人外周血单个核细胞HBV DNA的PCR检测

DETECTION OF HEPATITIS B VIRUS DNA IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF PATIENTS WITH CHRONIC HEPATITIS B BY PCR

1目的 探讨慢性乙型肝炎病人外周血单个核细胞 (PBMC)乙型肝炎病毒 (HBV) DNA和血清 HBVDNA的感染情况及其临床意义。 2方法 采用聚合酶链反应 (PCR)技术对 12 8例慢性乙型肝炎病人和 2 0例健康人进行了 PBMC及血清中 HBV DNA检测。 3结果 慢性乙型肝炎病人 PBMC中 HBV DNA的感染率为 6 4.2 % ,和血清 HBV DNA的感染率具有一致性倾向 (χ2 =0 .6 3,P>0 .0 5 ) ,慢性肝病病人 PBMC内 HBV DNA阳性率随肝病病情加重而升高。 4结论 PBMC中 HBV DNA整合在 HBV病毒传播和病情进程中起一定作用 ,检测 PBMC中 HBV DNA可作为判断病情和指导治疗的有用指标。

Objective\ To study the infection state of the HBV DNA in peripheral blood mononuclear cells(PBMC) and serum of patients with chronic hepatitis B and its clinical significance.\ Methods\ The HBV DNA in serum and PBMC of 128 cases with different degree infection of HBV and 20 healthy people were detected by polymerase chain reaction(PCR).\ Results\ The infection rate of HBV DNA in PBMC was 64.2%, which was of identical tendency with the infection rate of HBV DNA in PBMC and serum ( χ 2=0.63,P >0.05). The HBV DNA positive rate in PBMC was going higher along with the severity of hepatic disease.\ Conclusion\ The HBV DNA infection of PBMC may play a vital role in the occurence, transmission and process of hepatitis B virus. The detection of HBV DNA in PBMC of chronic hepatitic disease patients will help to determine the process of the disease and direct the therapy for the disease. [