无症状HBsAg携带者外周血淋巴细胞SCE变化

SISTER CHROMATID EXCHANGE IN PERIPHERAL LEUCOCYTES OF ASYMPOTOMATIC HBsAg CARRIERS

1目的 探讨乙型肝炎病毒 (HBV)感染对无症状 HBs Ag携带者遗传物质的损伤。 2方法 用常规法培养外周血淋巴细胞 ,用 5 -溴脱氧尿嘧啶核苷诱导姐妹染色单体互换 (SCE) ,用紫外线照射法制备 SCE标本 ,对2 0例无症状 HBs Ag携带者和 35例正常对照组的外周血淋巴细胞的 SCE频率进行了分析。3结果 无症状 HBs Ag携带者外周血淋巴细胞 SCE频率明显高于正常对照组 ,差异有极显著性 (t=3.10 1,P<0 .0 1)。 4结论 HBV感染可导致无症状 HBs Ag携带者的遗传物质不稳定 。

Objective\ To investigate the damage to the genetic material of the asymptomatic HBsAg carriers caused by the infection of hepatitis B virus(HBV).\ Methods\ The peripheral lymphocytes from 20 asymptomatic HBsAg carriers and 35 normal control individuals were cultured with the routine methods.The sister chromatid exchange(SCE)of the peripheral leucocytes was induced by BrdU and the SCE slides were prepared with the UPG method.\ Results\ The SCE frequency of the asymptomatic HBsAg carriers was significantly higher than that of the normal controls( t=3.101, P <0.01).\ Conclusion The infection of HBV may cause the instability of the genetic material of the HBsAg carriers and the instability may be related to the occurence of hepatocellular carcinoma. [