大豆低聚糖对肠道微生物的作用效应及各组分含量检测方法

Effects of Soybean Oligosaccharide on Intestinal Bacteria and Content Determination Method of Its Compositions

目的 :观察大豆低聚糖的肠道微生物的作用效应并建立一种大豆低聚糖各组分含量的检测方法。方法 :采用青春双歧杆菌和产气荚膜梭菌为肠道微生物指示菌 ;使用 μBondpak/CH氨基柱 ,高压液相色谱法 ,以纯水为洗脱液 ,分离大豆低聚糖各组分。结果 :大豆低聚糖能有效促进青春双歧杆菌在体外的增殖 ,而产气荚膜梭菌则几乎不能利用 ;HPLC法有效分离和准确测定大豆低聚糖中的各组份。结论 :大豆低聚糖是双歧杆菌的有效促生因子 ,HPLC方法是一种快速、准确、前处理简单、分离度高的含量检测方法。

Objective:To observe the effects of soybean oligosaccharide on intestinal bacteria and establish a content determination method of its compositions. Methods:Soybean oligosaccharide compositions were separated by HPLC on a μBondpak/CH column, with pure water as an eluent and Bifidobacterium adolescentis and Clostridium perfringens as indicator strains of intestinal bacteria.Results:Soybean oligosaccharide was effectively able to promote the growth of Bifidebacterium adolescentis in vitro, but didn't do for Clostridium perfringens. Meanwhile, its compositions were separated efficiently and determined accurately by HPLC. Conclusion:Soybean oligosaccharide is an efficient growth promoting factor of Bifidobacterium, and HPLC has the advantages of rapidity, simplicity for pretreatment, accuracy and good separation effect.