广东地区TTV感染情况及基因分型

Study on the prevalence and genotypes of TT virus Infection in Guangdong China

目的 调查广东地区TT病毒 (TTV)感染流行情况和基因分型。方法 选取TTVORF1的保守序列作内外引物 ,Ⅰ型、Ⅱ型同源性极高的序列nt2 46 4～nt2 5 2 0作包被探针 ,异源性大于 5 0 %的序列nt2 12 5～nt2 15 9作显色探针Ⅰ和显色探针Ⅱ ,采用微板核酸杂交 ELISA技术对 2 80例肝功能损害的肝炎患者血清进行TTV检测及TTV基因分型研究。结果 检测出 44例TTV阳性患者 ,检出率为 15 .71%。对 44例TTV阳性患者的标本进行分型 ,Ⅰ型、Ⅱ型和混合型比例为 30∶4∶10。慢性肝炎中TTV感染者与急性肝炎中TTV感染者比例为 2 8∶16。结论 在广东地区的肝炎疾病中 ,TTV有较高的发病率 ,且慢性肝炎感染TTV的机会多于急性肝炎 ,TTV可分为两个型 :Ⅰ型和Ⅱ型 ,Ⅰ型占 6 8.18% ,Ⅱ型占 9.0 9% ,Ⅰ型明显多于Ⅱ型。微板核酸杂交 ELISA技术特异性强 ,灵敏度高 ,分型准确快速 。

Objective To study the prevalence and genotypes of TT virus (TTV) infection in Guangdong China. Methods Two pairs of primers derived from open reading frame 1 (ORF 1) of the TT virus genome, capture probe from nt2464～nt2520, a conserved region of the TTV genome, and detector probe from nt2125～nt2159 were used to detect 280 sera samples of hepatitis patients by Microplate Sandwich Hybridization ELISA technique. Results Forty four (15.71%) of 280 hepatitis patients were positive for TTV DNA, 68.18% of which showed genotype Ⅰ infections, 9.09% showed genotype Ⅱ infections and 27.73% were mixed genotype Ⅰ/Ⅱ infections. There were 28 chronic hepatitis patients and 16 acute hepatitis patients in 44 TTV positive cases. Conclusion In Guangdong China, there was higher prevalence of TT virus infection in hepatitis patients, especially chronic hepatitis patients. There were two genotypes of TTV: 68.18% of genotype Ⅰ and 9.09% of genotype Ⅱ. Microplate sandwich hybridization ELISA technique is a sensitive and specific method in studying the prevalene and genotypes of TT virus.