高活性生淀粉糖化酶菌株F7的鉴定及其酶学性质的研究

Identification of High-active Raw Starch Glucoamylase Strain F7 and Study on Its Enzymatic Properties

对高活性生淀粉糖化酶菌株F7的归属和酶学性质进行了鉴定和探讨,从菌体形态特征和分子生物学特征方面对F7进行了鉴定,从F7的酶活力、酶对生淀粉的吸附力、最佳的作用温度、pH等方面探讨了F7的生淀粉糖化酶性质。结果表明:菌株F7归属藻菌纲(Phycomycetes),毛霉目(Mucorales),毛霉科(Mucoraceae),根霉属(Rhizopus Ehrenberg),米根霉种(Rhizopus oryzae)。其酶不仅对生淀粉吸附力大,消化能力强,而且其兼有的强酸性蛋白酶活力低;该酶作用pH范围是3.3～5.3;作用温度是35～45℃,酶活力稳定,在低温条件下酶液能保存较长时间;该酶对大米生淀粉的吸附率高,糖化能力也强。

The attribution of high-active raw starch glucoamylase strain F7 and its enzymatic properties are identified and discussed. The strain F7 is identified from bacteria morphology and molecular biolo- gy characteristics, its properties are explored from enzyme activity, absorption of raw starch enzyme, the optimum temperature and pH. The result indicates that the strain F7 belongs to Phycomycetes, Mucorales, Mucoraceae, Rhizopus Ehrenberg, Rhizopus oryzae. Not only is the absorption of raw starch enzyme big and the digestion strong, but also the strong acid protease activity is low. The study of glucoamylase produced by Rhizopus F7 shows that the optimal pH and temperature range for the enzyme reaction are 3.3~5.3 and 35~45℃ respectively. The enzyme is stable and can be stored for a long time at low temperature. The rate of absorption is proved to be high, saccharification ability is also strong.