富含多糖甜玉米幼穗RNA提取及高温胁迫基因表达分析

RNA Extracted from Polysaccharide-rich Sweet Corn Young Ears and the Analysis of Genes Quantitative Expression under Heat Stress

以改良Trizol试剂法从富含多糖的甜玉米幼穗中提取高质量、完整的总RNA,利用实时荧光定量PCR对高温胁迫下粤甜13雌穗发育的8个差异表达基因进行分析。结果表明,8个基因分为上调表达和下调表达,实时荧光定量PCR和测序法基因表达谱检测的基因上调或下调表达的趋势一致,上调表达基因ZM2G058057和下调表达基因ZM2G059964、ZM2G044670相对表达量较高,可能在高温胁迫影响甜玉米幼雌穗发育过程中起更重要的作用。

An improved Trizol method was used for extracting the total RNA from polysaccharide-rich young ears of sweet corn. The extract total RNA was high quality and integrity. Real-time quantitative PCR was used to investi- gate 8 genes expressions in ear development of cultivar Yuetianl3 under heat stress. The results indicated that 8 genes were divided into up- or down-regulated types, and their expression patterns by Real-time quantitative PCR were basically consistent with the digital gene expression data. Three of 8 genes, up-regulated expression gene ZM2G058057, down-regulated expression genes ZM2G059964 and ZM2G044670 had higher relative expression lev- els in ears, which may play very important roles in ears development under heat stress.