奥沙利铂长循环纳米脂质体对结肠癌细胞株体外作用研究

Study on Oxaliplatin long circulation nanoliposomes' effect on colon cancer cells in vitro

目的检测奥沙利铂长循环纳米脂质体药物样品对SW480结肠癌细胞株的毒性影响,并验证其长效性和缓控释作用。方法乙醇注入法制备出奥沙利铂长循环纳米脂质体药物样品,MTT实验检测其对SW480细胞株作用时效性与抑制率,并与标准品组对照分析。结果阳性对照组的IC50=70.96μg/mL,36 h内抑制率随着奥沙利铂浓度的增加迅速上升而达到顶峰。药物样品组的IC50=85.39μg/mL,72 h内抑制率随着药物样品浓度的增加而稳步上升。结论奥沙利铂长循环纳米脂质体药物样品IC50=85.39μg/mL,抑制率随着浓度的增加而上升且增势稳定,100μg/mL以上组别的抑制率在72 h后均达到90%以上,具有长效性,缓释稳定性,其生物利用度更优。

【Objective】 To detect LCNL-Oxaliplatin＇s toxic effect for SW480 colon cancer cells,and verify the stability and long-term sustainability.【Methods】 Prepared LCNL-Oxaliplatin＇s drug samples by ethanol injection,Cell MTT detected the toxic effect for SW480 colon cancer cells,compared the stability and longterm sustainability with positive groups.【Results】 Cell MTT results showed that the positive groups＇ IC50 = 70.96 μg/mL,and the LCNL-Oxaliplatin groups＇ IC50=85.39 μg/mL,the inhibition rate of the positive groups have rising with Oxaliplatin＇s concentration in 36 hours,and the LCNL-Oxaliplatin groups steadily released within 72 hours.【Conclusion】 The LCNL-Oxaliplatin groups＇ IC50 =85.39 μg/mL,show the inhibition rate steadily increase with drug concentration.Drug concentration more than 100 μg/mL groups＇ inhibition rate reached above 90% after 72 hours,have showed better control ability,stability and long-term sustainability than the positive groups.