大鼠慢性非细菌性前列腺炎模型中调节性T淋巴细胞表达的变化及意义

Change and significance of the regulatory T lymphocytes expression in rats model of chronic abacterial prostatitis

目的探讨调节性T淋巴细胞在大鼠慢性非细菌性前列腺炎模型外周血中的变化及意义。方法2011年12月至2012年7月，390g左右的Wistar雄性大鼠12只采用简单随机抽样法平均分为实验组和对照组。实验组大鼠行去势术，术后皮下注射17-β雌二醇（0．25mg／d，连续30d）建立慢性非细菌性前列腺炎模型，对照组大鼠饲养30d后同实验组一起处理。建模成功后，用流式细胞仪检测大鼠外周血中CD4＋CD25＋T淋巴细胞群比例和CD8＋CD28-T淋巴细胞群比例的变化。结果实验组CD4＋CD25＋T淋巴细胞群比例（7．90±1．74）％与对照组（11．63±1．36）％相比明显下降（P〈0．01）。实验组CD8＋CD28-T淋巴细胞群比例（17．18±2．83）％与对照组（24．64±4．76）％相比也有所下降，差异有统计学意义（P〈0．01）。结论大鼠慢性非细菌性前列腺炎模型外周血中cD：cD＋5T淋巴细胞群比例和CD8＋CD28-T淋巴细胞群比例的变化可能为调节性T淋巴细胞参与大鼠慢性非细菌性前列腺炎的致病机制提供证据。、

Objective To investigate the change and significance of regulatory T lymphocytes in pe- ripheral blood of rats model of chronic abacterial prostatitis. Methods Twelve Wistar rats with weight of approximate 390 g were randomly divided into two groups, model group and control group. Rats in the model group was injected subcutaneously 17β-estradiol（0.25 rag/day, for 30 days） after castration to establish rat model of chronic abacterial prostatitis. Flow cytometry was applied to detect the frequency of CD4＋ CD25＋T cells and CD8＋CD28-T cells in peripheral blood of rats after model establishment. Results Compared with control group （ 11.63± 1.36） %, the proportion of CD4＋ CD25＋T T lymphocytes in model group （ 7.90± 1.74） % significanhy decreased （P〈0.01）. Compared with control group （24.64±4.76）%, the proportion of CD8＋CD28-T lymphocytes in model group （17.18±2.83）% also significantly decreased （P〈0.01）. Conclusions The changes of the ratio of CD4＋ CD25＋T lymphocytes and CD8＋CD28-T lymphocytes in peripheral blood of rats model of chronic abacterial prostatitis provided evidences for pathogenic mechanism of regulatory T lymphocytes participating in the development of chronic abaeterial prostatitis.