盐酸贝西沙星滴眼液中苯扎氯铵的HPLC法测定

Determination of Benzalkonium Chloride in Besifloxacin Hydrochloride Eye Drops by HPLC

建立了高效液相色谱法测定盐酸贝西沙星滴眼液中苯扎氯铵的含量。采用Kromasil C18柱,5 mmol/L乙酸铵溶液(含1%三乙胺,用冰乙酸调至pH 5.2±0.5)-乙腈(29∶71)为流动相,检测波长为215 nm,流速1.0 ml/min。苯扎氯铵在5～15 g/ml浓度范围内线性关系良好(R2=0.998 3),平均回收率为99.4%(RSD=0.42%)。

An HPLC method was established for the determination of benzalkonium chloride in besifloxacin hydrochloride eye drops. A Kromasil C18 column was used, with the mobile phase of 5 mmol/L ammonium acetate （containing 1% triethylamine, adjust pH to 5.2±0.5 with glacial acetic acid）-acetonitrile （29： 71）, at the detection wavelength of 215 nm. The flow rate was 1.0 ml/min. The calibration curve of benzalkonium chloride was linear in the concentration range of 5 - 15 μg/ml （R2=0.998 3）. The average recovery was 99.4 % with RSD of 0.42 %.