飞秒激光对人类细胞内钙信号的调控作用

Calcium Signal Modulation of Human Cells by Femtosecond Laser

飞秒激光以其独特和优良的光学性质,为生物光子学的研究带来了全新的研究领域和技术手段。在细胞和分子生物学的研究中,飞秒激光可以精确地在亚细胞或衍射极限水平上对细胞实现精确的刺激和操作。对于飞秒激光调控人类细胞内钙信号这一全新的现象进行了回顾和综述,讨论了飞秒激光释放细胞钙存储的可能机制,分析了飞秒激光释放细胞钙信号的物理和生理过程,在细胞生物学和分子生物学的水平上探讨了细胞对飞秒激光刺激的应激反应可能的原理,并阐述了由此而来的一系列后续分子和生理过程。这种纯光学的分子信号调节对于相关细胞信号通路的研究具有重要意义,并有望进一步应用于基于飞秒激光的基因表达调节和干细胞分化的研究。

By their unique and excellent optical properties, femtosecond lasers have brought novel developments and methodologies in biophotonics researches. In researches of cell and molecular biology, femtosecond lasers can perform precise stimulations and operations to cells at the subcellular or diffraction limit level. In this article, calcium signal modulation in human cells by femtosecond lasers is reviewed, possible mechanism of optical release of cellular calcium store is discussed, and physical and physiological processes of the release are analyzed. The hypothesis of cellular responses to femtosecond lasers is investigated at cellular and molecular level, with demonstrations of following molecular and physiological processes. This all-optical modulation of molecular signals is very important to the researches of cellular signaling pathways, which can be further applied in optical regulation of gene expression and stem-cell differentiation.