摘要
目的 :研究微生物活细胞在笔者研制的电化学生物传感器上的响应 ,探讨使用该生物传感器系统进行微生物活细胞快速测量的可能。方法 :设计、研制了能将微生物活细胞新陈代谢产生的电子转移转换为电流输出的电化学生物传感器 ,并设计、研制了单片计算机控制、带恒温测量池的专用测定仪 ,研究了传感器对大肠杆菌、啤酒酵母、霍乱弧菌及卡介菌苗的响应 ,并实际测量了发酵罐中啤酒酵母菌总数及消毒鲜牛奶中的细菌总数。结果 :传感器的电流输出反映了微生物活细胞新陈代谢的程度和能力。结论 :研制的生物传感器系统可用于菌种和培养基组分的快速筛选、微生物活细胞生长特性的快速评估 。
Objective: To study the responses of the electrochemical biosensor system for analyzing microbes to determine whether the system can be used to analyze microbes rapidly. Methods: The electrochemical biosensor system had been developed and the responses to colifome, brewage microzyme, comma bacillus and bcg vaccine had been observed. Results: The current measured from biosensor reflected the metabolism of microbes. Conclusion: The system not only could pick upon the optimum strain on fixed culture medium or the optimum substrate to special strain fleetly but also could be used to evaluate the microbe growth character apace, monitor zymogen upgrowth in fermentor and determine total number of bacteria in fresh creamery rapidly.
出处
《华夏医学》
2000年第3期252-255,共4页
Acta Medicinae Sinica
基金
广西科技厅攻关项目基金资助课题!桂科攻9817105
